We narrowed to 7,518 results for: Lif

1. TurboRFP-pBAD

   Plasmid

   #54858

   Purpose

   Localization: Protein Expression Vector , Excitation: 553, Emission: 574

   Depositor

   Dmitriy Chudakov, Michael Davidson, Dorus Gadella

   Article

   Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

   Type

   Empty backbone

   Use

   Tags

   6xHis and TurboRFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pHEE901

   Plasmid

   #91707

   Purpose

   CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV MDM-2

   Plasmid

   #16441

   Depositor

   Bert Vogelstein

   Article

   Oliner et al Nature. 1992 Jul 2. 358(6381):80-3.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSIIbsr-EKARrEV-NLS

   Plasmid

   #173854

   Purpose

   A lentiviral vector for EKARrEV-NLS

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   EKARrEV-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS SK mCherryROSAegfp

   Plasmid

   #54322

   Purpose

   Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

   Depositor

   Silvestro Conticello

   Article

   Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

   Insert

   mCherry and EGFP
   

   Use

   CRISPR, Mouse Targeting, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX-UBC_NKX2-1_Short_V5-GFP

   Plasmid

   #221499

   Purpose

   NKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5-linker-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3789 pmko-neo RNAi-GFP

   Plasmid

   #8881

   Depositor

   Stanley Korsmeyer

   Article

   Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

   Insert

   GFP
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pWPI-TFDP1

   Plasmid

   #114299

   Purpose

   Constitutive expression of the TFDP1 protein

   Depositor

   Patrick Salmon

   Article

   Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

   Insert

   TFDP1 (TFDP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

   Plasmid

   #182009

   Purpose

   CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   IgKchL_HA_STf_myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.27_GRHL-SPE

    Plasmid

    #177774

    Purpose

    Luciferase reporter for GRHL

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLS.491

    Plasmid

    #184956

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C

    Promoter

    T7/lac

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSETA-qmTQ2-citrate

    Plasmid

    #225251

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-citrate
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPI138

    Plasmid

    #113229

    Purpose

    extrachromosomal expression vector, expression of LifeAct-GFP

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    LifeAct
    

    Use

    Dictyostelium discoideum expression vector, extra…

    Tags

    GFP

    Expression

    Mutation

    Promoter

    act15

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_NES_STf

    Plasmid

    #182001

    Purpose

    CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    NES-STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDM8 hCD81

    Plasmid

    #11588

    Depositor

    Shoshana Levy

    Article

    Oren et al Mol Cell Biol. 1990 Aug . 10(8):4007-15.

    Insert

    CD81 (CD81 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3 CDK7 D155A HA (P#812)

    Plasmid

    #14648

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

    Insert

    Cdk7 (CDK7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D155A: dominant negative

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

17. AAV-FLEX-CAG-CyRFP1

    Plasmid

    #84357

    Purpose

    Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent)

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    CyRFP1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Double FLEXed

    Promoter

    CAG

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCL.139

    Plasmid

    #184994

    Purpose

    Express Cas9-P2A-Eco1RT in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1/8-NORAD-(A)4x

    Plasmid

    #199209

    Purpose

    1/8 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Esther Braselmann

    Article

    Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

    Insert

    1/8-NORAD
    

    Use

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. p153 pPr-cre

    Plasmid

    #8403

    Purpose

    Expression of Cre from tissue-specific, androgen-inducible probasin promoter.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    probasin driven Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    probasin promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

21. CAG-loxP-3xpA-loxP-H2B-sfGFP

    Plasmid

    #136064

    Purpose

    H2B-sfGFP cre reporter

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    HIST1H2BB (H2BC3 Human)
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 1/2-NORAD-(A)4x

    Plasmid

    #199208

    Purpose

    1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Esther Braselmann

    Article

    Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

    Insert

    1/2-NORAD
    

    Use

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pXWJ109Hg-gfp

    Plasmid

    #123148

    Purpose

    A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mMaroon-PAK-mMaroon

    Plasmid

    #84361

    Purpose

    FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-PAK-mMaroon
    

    Use

    Tags

    mMaroon flanking PAK domain on N and C terminus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBMN mCherry-TAX1BP1(ΔUBD)

    Plasmid

    #119689

    Purpose

    For mammalian expression of TAX1BP1 without its UBD

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    UBD deletion (1-755)

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. As4

    Plasmid

    #123161

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J115-30arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J115-30arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J115, ParsR

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PXWABS62(E11-TEV)

    Plasmid

    #123157

    Purpose

    For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ParsR-ABS62, Pecf11

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. mMaroon-RTK-mMaroon

    Plasmid

    #84359

    Purpose

    FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-Rtk--mMaroon
    

    Use

    Tags

    mMaroon flanking Rtk domain on N and C terminus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-myrSNAP

    Plasmid

    #60098

    Purpose

    Expresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditional

    Depositor

    Gregory Jefferis

    Article

    Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

    Inserts

    SNAP-tag
    histone-GFP
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA4TO-Kif18b-24xGCN4_v1

    Plasmid

    #72228

    Purpose

    A fusion of the Kif18b motor protein to the v1 SunTag peptide array

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Kif18b (KIF18B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge