We narrowed to 10,838 results for: ENA

1. V5-LOV-Turbo-ERM

   Plasmid

   #199664

   Purpose

   expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   SEC61B and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDIRECT_22A

   Plasmid

   #91133

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe-puro-RIPK3

   Plasmid

   #78829

   Purpose

   retroviral expression of RIPK3

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (RIPK3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   5'LTR

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pET24a-ncOGT-FL

   Plasmid

   #190821

   Purpose

   For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E.coli

   Promoter

   T7

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. PB-SRT-tdTomato

   Plasmid

   #154885

   Purpose

   piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   piggyBac SRT
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDB179-TfCut2-S121P-D174S-D204P

   Plasmid

   #218695

   Purpose

   Encodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   TfCut2
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   S121P-D174S-D204P

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-SRT-Puro

   Plasmid

   #154884

   Purpose

   piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   piggyBac SRT
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

   Plasmid

   #116885

   Purpose

   FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RVH)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbi_RP_2XMTS-MicroID2-FLAG

   Plasmid

   #188524

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   2XMTS-MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. RFP Dynamin2 Wild Type

    Plasmid

    #128152

    Purpose

    Expression of Dynamin 2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

    Insert

    Dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

    Plasmid

    #117910

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R44A on eDHFR

    Promoter

    T7

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-cytoEKAR4

    Plasmid

    #174438

    Purpose

    Cytosol-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    cytoEKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRDA_722

    Plasmid

    #216077

    Purpose

    CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Destination

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HA-RIPK3

    Plasmid

    #78804

    Purpose

    To overexpress RIPK3 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-EF1a-i53

    Plasmid

    #216243

    Purpose

    Express i53 in mammalian cells as a HDR enhancer

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    i53
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-pmEKAR4

    Plasmid

    #174440

    Purpose

    Plasma membrane-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP1356

    Plasmid

    #119276

    Purpose

    for cloning new sgRNAs

    Depositor

    Jason Peters, Carol Gross, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG Cas9-2A-Citrine

    Plasmid

    #92358

    Purpose

    CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9 2A Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

    Plasmid

    #125228

    Purpose

    Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRDB_161

    Plasmid

    #216091

    Purpose

    Cas9 [Sp] CRISPRi targeting CD81, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD81 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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