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SOX2se-CPG-TV

Plasmid

#70155

Purpose

a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se

Use

Mouse Targeting and Synthetic Biology

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTH

Plasmid

#178949

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTH-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K(AAA)12-P2A-RFP

Plasmid

#105687

Purpose

Double fluorescent stalling reporter K(AAA)12

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)12
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-human Igf2bp2-IRES-GFP

Plasmid

#91890

Purpose

Retroviral expression of human Igfbp2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Igf2bp2 (IGF2BP2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD

Plasmid

#81053

Purpose

bacterial expression of the catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ eiF4A2 myc HIS

Plasmid

#71658

Purpose

mammalian expression of eiF4A2

Depositor

Gideon Dreyfuss

Insert

EIF4A2 (EIF4A2 Human)

Tags

HIS and myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
Blimp1pGL3 (Human Blimp1 promoter in pGL3-basic)

Plasmid

#40340

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

Blimp1 promoter (PRDM1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

Blimp1

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pLNCX1 HA Akt1

Plasmid

#15990

Depositor

Morris Birnbaum

Article

Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

Insert

Akt1 (Akt1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
YFP-Rac2

Plasmid

#11393

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Tags

YFP

Expression

Mammalian

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pJW1310

Plasmid

#61253

Purpose

Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

U6 promoter

Use

CRISPR; Cloning template

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt5a

Plasmid

#42279

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt5a (Wnt5a Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pUB_smFLAG_KDM5B_MS2

Plasmid

#81084

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_KDM5B_24xMS2

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-Tev

Plasmid

#177142

Purpose

Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

Tev

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f2

Plasmid

#158756

Purpose

Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f2

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9

Plasmid

#110823

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-Ii

Plasmid

#65313

Purpose

Cytoplasmic ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Use

Lentiviral

Mutation

Streptavidin in its cytoplasmic part

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

Plasmid

#86885

Purpose

C-terminal part of the split-BioID

Depositor

Mathieu Bollen

Article

Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

Insert

BirA* (AA141-321)- HA- NIPP1]

Tags

HA tag

Expression

Mammalian

Mutation

BirA Amino Acids 141-321

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1 H83L H86L

Plasmid

#12211

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

PBD (p21-binding domain) mutant: H83L, H86L (see …

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pMTTH+H1.4

Plasmid

#157796

Purpose

Expression of human linker histone H1.4

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4 (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-gamma-tubulin

Plasmid

#87956

Purpose

Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene …

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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