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We narrowed to 12,028 results for: LEA

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Showing: 3001 - 3020 of 12028 results

  1. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80059
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80059-plasmid-map-sequence-id-152593

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  2. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid
    #79886
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Ubiquitin
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79886-plasmid-map-sequence-id-150062

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  4. TALEN-CCR5R18-CtermQ7

    Plasmid
    #51443
    Purpose
    Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain
    Depositor
    David Liu
    Article
    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:
    Insert
    TALEN CCR5 Right (R18) Q7 C-term
    Use
    TALEN
    Tags
    3xFLAG and Fok1 KK variant
    Expression
    Mammalian
    Mutation
    K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q
    Promoter
    CMV
    Available Since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51443-plasmid-map-sequence-id-78480

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  14. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

    Plasmid
    #169077
    Purpose
    Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII
    Depositor
    Sascha Martens
    Article
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Tags
    GFP tag, GGlinker, TEV cleavage site. StrepII and…
    Expression
    Bacterial and Insect
    Promoter
    Polyhedrin promoter
    Available Since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169077-plasmid-map-sequence-id-332050

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  19. DiLiCre 2.0

    Plasmid
    #198752
    Purpose
    A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    Cre-PhoCI-ERT2-ERT2
    Use
    Lentiviral
    Tags
    Cre, kept in a photocleavable site, followed by 2…
    Expression
    Bacterial and Mammalian
    Promoter
    TRE3GV
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198752-plasmid-map-sequence-id-397647

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Showing: 3001 - 3020 of 12028 results