We narrowed to 12,214 results for: LEA
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Plasmid#108859PurposeFnCpf1 Gateway entry plasmidDepositorInsertFnCpf1
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationCpf1 is rice codon optimizedAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGG-NFIX2
Plasmid#112699PurposeExpression of NFIX2 protein and GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNuclear Factor I X2 (Nfix Mouse)
TagsHAExpressionMammalianAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
NKP38 GFP (1009)
Plasmid#62021Purposemammalian expression of nuclear envelope transmembrane proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAYTL1 (LPCAT2 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET26b-Granzyme A (S-A)
Plasmid#8825DepositorInsertHuman Granzyme A inactive (GZMA Human)
Tags(His)6ExpressionBacterialMutationchanged Serine 184 to AlanineAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-nlsSED1
Plasmid#178190PurposeSED1 biosensor for expression in Arabidopsis thalianaDepositorInsertSED1 biosensor
TagsSV40 NLSExpressionPlantPromoterpUBQ10Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP
Plasmid#45578PurposeExpresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorPublicationInsertcoY2 Ani
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3-K230-FokI_L (wt)
Plasmid#32544DepositorInsertZFN
TagsHA epitopeExpressionMammalianPromoterCMVAvailable sinceDec. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 prescission LIC cloning vector (HPKS)
Plasmid#29720DepositorTypeEmpty backboneTags6xHis and Prescission cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTE4999
Plasmid#107555PurposeExpresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthFnCpf1(dead1)
UseCRISPRTags3xHA and NLSExpressionMammalianMutationD917APromoterCMVAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN6kwh
Plasmid#44724DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCMV-D2i promoter
htetR::NLS
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre
Plasmid#112683PurposeAn AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinaseDepositorInsertiRFP713
UseAAVTags2A-iCre and Nuclear localization signalPromoterCMV-IEAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
NET29A.Hs pEGFP-N2 (1181)
Plasmid#62040Purposemammalian expression of nuclear envelope transmembrane proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNET29A (TMEM120A Human)
TagsEGFPExpressionMammalianMutationSplice variantPromoterCMVAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
UBH14
Plasmid#28158PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertE3 ligase UHRF2 (UHRF2 Human)
TagsHis tag with thrombin cleavageExpressionBacterialMutationSRA Domain (residues 419-648)Available sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXcX-PRS-EGFP
Plasmid#25865DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPRS driven enhanced green fluorescent protein
ExpressionMammalianAvailable sinceAug. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACt-mCherry-NLS
Plasmid#138214PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsACt-mCherry-NLS (Adcy10 Rat)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-sACt-mCherry-NES
Plasmid#138215PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsACt-mCherry-NES (Adcy10 Rat)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_S94A-PolyA
Plasmid#112286PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Serine 94 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Non-specific sgRNA
Plasmid#109432PurposeMLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNon-specific gRNA
UseCRISPRExpressionMammalianPromoterhU6Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only