We narrowed to 11,275 results for: VARS

1. pAB078d5

   Plasmid

   #79204

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ppro1 434cI
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR V2 sgIREB2_2

   Plasmid

   #169886

   Purpose

   Disrupt IREB2

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   sgRNA targeting IREB2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PKAcs-GGSD5X-GFP

   Plasmid

   #108584

   Purpose

   Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

   Depositor

   Robert Hughes

   Article

   Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

   Insert

   protein kinase A, catalytic subunit
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H87Q and W196R

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSTAR-mOrange2-sfGFP

   Plasmid

   #115693

   Purpose

   Generation of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent Timer

   Depositor

   David Suter

   Article

   Alber et al Mol Cell. 2018 Aug 17. pii: S1097-2765(1

   Insert

   SA-mOrange2-sfGFP-SD
   

   Use

   Lentiviral

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p416 25Q GPD

   Plasmid

   #1177

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW)

   Plasmid

   #189791

   Purpose

   Express wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

   Depositor

   Robert Singer

   Article

   Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

   Insert

   beta-globin (HBB Mouse, Human)
   

   Expression

   Mammalian

   Promoter

   Ponasterone A inducible

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB094a

   Plasmid

   #79241

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PBAD rpoZ-HA4; PC araC
   

   Expression

   Bacterial

   Promoter

   PBAD; PC

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiGuide_CjCas9-Puro

   Plasmid

   #202564

   Purpose

   hU6-BsmBI-gRNA-Puro

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   CjCas9 sgRNA cassette
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2T-CMV-evoCjCas9-TadCDd-BlastR

   Plasmid

   #202558

   Purpose

   Mammalian expression of evoCjCas9 TadCDd

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-TadCDd
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3 mSmoothened WT

    Plasmid

    #126408

    Purpose

    Full length mouse Smoothened cDNA. Expression of Smoothened in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

    Insert

    m-Smoothened (Smo Mouse)
    

    Expression

    Mammalian

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p416 103Q GPD

    Plasmid

    #1180

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

12. LEPG

    Plasmid

    #111160

    Purpose

    miR-E (miR-30 variant)-based RNAi with GFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Mutation

    WT

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-UGI-nCas9(H840A)-GSVG

    Plasmid

    #187411

    Purpose

    DddA GSVG variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(-)-mIFNa2

    Plasmid

    #135098

    Purpose

    Protein expression of mouse IFNa2 in mammalian cells

    Depositor

    Pablo Penaloza-MacMaster

    Article

    Palacio et al J Exp Med. 2020 Dec 7;217(12). pii: 1520

    Insert

    mIFN-α2 (Ifna2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid

    #115698

    Purpose

    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

    Depositor

    Eckhard Boles

    Article

    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

    Insert

    SSKar2-sfpHluorin-HDEL
    

    Tags

    ER retention signal containing HDEL and ER signal…

    Expression

    Yeast

    Mutation

    for introduced mutations see publication

    Promoter

    MET25

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDJSVT86

    Plasmid

    #108965

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

    Type

    Empty backbone

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-flex-oChIEF-citrine

    Plasmid

    #50973

    Purpose

    flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Mutation

    inverted and inserted between two lox sites

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a(+)- PC-MjMAT

    Plasmid

    #179372

    Purpose

    PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

    Depositor

    Andrea Rentmeister

    Article

    Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

    Insert

    PC-MjMAT
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L147A, I351A

    Promoter

    T7

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p416 72Q GPD

    Plasmid

    #1179

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 72Q (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

    Plasmid

    #159853

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mNeon(PATC)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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