We narrowed to 3,916 results for: LYC

1. ZsGreen1-cMYC/pLVX-Puromycin

   Plasmid

   #180278

   Purpose

   Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

   Depositor

   Andre Catic

   Article

   Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

   Insert

   ZsGreen1-cMYC fusion protein MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MGAT2-RpHLuorin2

   Plasmid

   #171718

   Purpose

   Cis-/medial-Golgi expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
   

   Use

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-GRAM-H

   Plasmid

   #211700

   Purpose

   Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (EGFP-GRAM1b G187L) in mammalian cells

   Depositor

   Yasunori Saheki

   Article

   Ercan et al EMBO J. 2021 Mar 15;40(6):e106524. doi:

   Insert

   GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Leucine

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR v2-dCas9

   Plasmid

   #112233

   Purpose

   Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

   Depositor

   Thomas Gilmore

   Article

   Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

   Inserts

   dCas9
   Porumycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed: Aspartic acid 10 to Glycine, Histidine 8…

   Promoter

   EFS-NS

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST53-LRRK2-G2019S

   Plasmid

   #25045

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Glycine 2019 mutated to Serine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1-EGFP-GRAM-W

   Plasmid

   #211704

   Purpose

   Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Tryptophan

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETcon_SARS-CoV-2_RBD

   Plasmid

   #166782

   Purpose

   Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

   Depositor

   Jesse Bloom

   Article

   Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

   Insert

   Spike receptor binding domain (RBD) from SARS-CoV-2 (S )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-TREtight-mTagBFP2-B19G

   Plasmid

   #100799

   Purpose

   helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Has Service

   AAV1

   Insert

   mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CoV2-Spike-EF1a-D614G-N501Y

   Plasmid

   #177939

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (D614G, N501Y) (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G, N501Y

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. B-GRAM-W

    Plasmid

    #211707

    Purpose

    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Tryptophan

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. B-GRAM-H

    Plasmid

    #211706

    Purpose

    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGES401

    Plasmid

    #190198

    Purpose

    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRR-AR

    Plasmid

    #73045

    Purpose

    Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)

    Depositor

    David McMillen

    Article

    Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

    Insert

    Androgen receptor (AR Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    please see depositor comments below

    Promoter

    glyceraldehyde phosphate dehydrogenase (GPD)

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1 Utr230-EGFP-3XNLS

    Plasmid

    #58466

    Purpose

    Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Inserts

    Utr230 with 3X glycine linker (UTRN Human)
    3X NLS with 3X stop codons
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1-B-GRAM-W-NeoR

    Plasmid

    #211710

    Purpose

    Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Tryptophan

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLJM1-EGFP-GRAM-H

    Plasmid

    #211703

    Purpose

    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (pLJM1-EGFP-GRAM1b G187L)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB_NE1m

    Plasmid

    #123308

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE sensor GRAB_NE1m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    contains a glycine-to-threonine mutation at posit…

    Promoter

    hSyn

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHIV-EGFP.Flag-EZH2

    Plasmid

    #220243

    Purpose

    Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPSV39-ugi

    Plasmid

    #186556

    Purpose

    Broad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    ugi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-CMV GFP-LC3B G120A

    Plasmid

    #123115

    Purpose

    Expresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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