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Addgene

We narrowed to 13,007 results for: cdna

Showing: 6041 - 6080 of 13007 results
  1. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Tags
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. cDNA5-FRT/TO-APT2-LAMA-F98

    Plasmid
    #234427
    Purpose
    Express APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position F98
    Depositor
    Insert
    APT2 fused to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position F98 (LYPLA2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA5-FRT/TO-APT2-LAMA-G97

    Plasmid
    #234426
    Purpose
    Express APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97
    Depositor
    Insert
    APT2 fused to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97 (LYPLA2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. PME-1 S156A (inakt.mut) pcDNA-StrepIII

    Plasmid
    #119293
    Purpose
    Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the methylesterase active center
    Depositor
    Insert
    PPME1 (PPME1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S156A
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

    Plasmid
    #233064
    Purpose
    Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.
    Depositor
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Use
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

    Plasmid
    #233063
    Purpose
    Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.
    Depositor
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Use
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Threonine 923 to Glycine
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA5/FRT/TO_eGFP-pScaC-MTS

    Plasmid
    #228827
    Purpose
    For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells
    Depositor
    Insert
    ScaC
    Use
    Tags
    MTS and eGFP
    Expression
    Mammalian
    Mutation
    aa 33-223
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 6041 - 6080 of 13007 results