We narrowed to 17,391 results for: puro

1. HA-HIF2α P405A/P531A/N847A-pBabe-Puro

   Plasmid

   #25956

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Promoter

   Available since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

   Plasmid

   #162337

   Purpose

   Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6; mU6

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-Tight-Puro FLAG-SMAD4 TetON

   Plasmid

   #107129

   Purpose

   Inducible expression of Flag-tagged SMAD4 in mammalian cells

   Depositor

   Joan Massague

   Article

   David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

   Insert

   SMAD4
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-ProtC

   Plasmid

   #162099

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-StrepTagII-ProtC
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-ProtC

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

   Plasmid

   #123360

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   Empty LbCpf1 crRNA cloning cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

   Plasmid

   #202568

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

   Plasmid

   #157986

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV-MRLC1-mRuby2-IRES-PuroR

   Plasmid

   #103031

   Purpose

   Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti PGK mKate2-OGT 5N5A (Puro)

   Plasmid

   #154293

   Purpose

   Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x FLAG and mKate2

   Expression

   Mammalian

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations; HCF-…

   Promoter

   PGK

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2

    Plasmid

    #117406

    Purpose

    SpyCas9 sgRNA 2 targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SpyCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-uORF-HA-birA*-STOP-IRES-Puro

    Plasmid

    #120558

    Purpose

    Expresses control HA-birA* in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    birA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-EF1a-CDK6-mutPAM-PGK-puro

    Plasmid

    #187638

    Purpose

    sg resistant CDK6 ORF

    Depositor

    William Kaelin

    Article

    Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    PAM site mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-Myc-PGK-Puro-IRES-GFP

    Plasmid

    #75124

    Purpose

    Retroviral expression plasmid encoding wild type murine Myc

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

14. N113 nLV EF-1a-Gal4-Stop-PGK-Puro

    Plasmid

    #44176

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    Gal4/DBD (1-147)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCT-CMV-UBE3B(C1036A)-copGFP-Puro

    Plasmid

    #107344

    Purpose

    Expresses UBE3B mutant C1036A fused to copGFP in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3B(C1036A)copGFP (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    copGFP

    Expression

    Mammalian

    Mutation

    mutation C1036A

    Promoter

    CMV

    Available since

    April 19, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLenti CMV Puro DEST (w118-1) MXRA7

    Plasmid

    #107500

    Purpose

    Expresses MXRA7, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    MXRA7 (codon optimized) (MXRA7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177433

    Purpose

    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.

    Depositor

    David Andrews

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

    Plasmid

    #22034

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The LOV domain contains the mutation C450M.Rac1 s…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

    Plasmid

    #204547

    Purpose

    Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deltaUBA

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-PGK-flag-RAP1B-V12-puro

    Plasmid

    #156168

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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