165,065 results

1. pCAG-tTA

   Plasmid

   #104102

   Purpose

   Expresses tTA2 under the CAG promoter in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

   Insert

   tTA2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBKT7-GW

   Plasmid

   #61703

   Purpose

   Gateway compatible Y2H vector - BD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJC6-EV

   Plasmid

   #163454

   Purpose

   empty vector control for pLJC6

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-RFP

   Plasmid

   #22910

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Insert

   Chicken Beta-actin promoter driving DsRedExpress
   

   Use

   AAV; Adeno-associated virus

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHN922

   Plasmid

   #178975

   Purpose

   Expression of GST-His8-GFP-nanobody

   Depositor

   Kazuhiro Abe

   Insert

   GST-His8-GFP-nanobody
   

   Use

   Affinity Reagent/ Antibody

   Tags

   GST, His8

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Laconic/pcDNA3.1(-)

   Plasmid

   #44238

   Purpose

   a genetically-encoded sensor for lactate concentration, transport and metabolic flux

   Depositor

   Luis Felipe Barros

   Article

   San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

   Insert

   Laconic (LACtate Optical Nano Indicator from CECs)
   

   Use

   Lactate biosensor

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWPI-N1ICD

   Plasmid

   #185525

   Purpose

   Constitutive overexpression of human NOTCH1 intracellular domain

   Depositor

   Sean Palecek, Eric Shusta

   Article

   Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

   Insert

   NOTCH1 intracellular domain (NOTCH1 Human)
   

   Use

   Lentiviral

   Promoter

   EF-1a

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-CMV-N-mCherry

   Plasmid

   #123215

   Purpose

   N-terminal mCherry tag. Gateway destination vector for mammalian expression.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-LaminA-C-18

   Plasmid

   #55068

   Purpose

   Localization: Nuclear Envelope, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Lamin A (LMNA Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-SEpHluorin

    Plasmid

    #32001

    Depositor

    Sergio Grinstein

    Article

    Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

    Insert

    mCherry
    

    Tags

    Superecliptic pHluorin

    Expression

    Mammalian

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

    Plasmid

    #60903

    Purpose

    Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Cas9 dead
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available Since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-GFP

    Plasmid

    #11150

    Purpose

    Mammalian expression vector for expression of GFP (CAG promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    EGFP from Aequorea victoria
    

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNAintron-EBOV-GP

    Plasmid

    #215692

    Purpose

    Produces the Ebola virus glycoprotein

    Depositor

    Melinda Brindley

    Article

    Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

    Insert

    EBOV-GP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pEGFP-N1-MITF-M

    Plasmid

    #38131

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-M (MITF Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDualEX-SMN2

    Plasmid

    #214431

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-G-Creon shRNA[Control]

    Plasmid

    #181824

    Purpose

    This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

    Depositor

    Eun Mi Hwang

    Article

    Kim et al J Neurosci Methods. 2021 Dec 21:109452.

    Type

    Empty backbone

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    EF1a/U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti CMV/TO Hygro empty (w214-1)

    Plasmid

    #17484

    Purpose

    3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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