We narrowed to 7,518 results for: Lif
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pSLS.546
Plasmid#184963PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
UseTagsExpressionBacterialMutationpriB donor G1069A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-STf
Plasmid#182008PurposeCMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell linesDepositorInsertLyn11-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPO640
Plasmid#195296PurposepTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).DepositorInsertminiMcCAST (Tn7575) with KanR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET5
Plasmid#107437PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET5 (MET5 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …PromoterGAL1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIhh-5A9-LUC
Plasmid#46318DepositorInsert5 copies of A9 sequence from Ihh promoter
UseTagsluciferaseExpressionMammalianMutationPromotermouse osteocalcin gene 2 promoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLS.540
Plasmid#184959PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
UseTagsExpressionBacterialMutationfabH donor G954A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSopBC460S
Plasmid#112514PurposePsopB driven sopB (catalytically inactive) and sigEDepositorInsertsopB (catalytically inactive)/sigE operon
UseTagsExpressionBacterialMutationSopB C460SPromoterPsopBAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-NBR1(ΔUBD)
Plasmid#119691PurposeFor mammalian expression of NBR1 without its UBDDepositorInsertNBR1 (NBR1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationUBD deletion (1-906)PromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pKC123
Plasmid#134240Purposeexpresses gRNA targeting CENP A 3'DepositorInsertCENP A 3' CRISPR construct
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
Plasmid#128167PurposeMonomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, GSAx9 linker)DepositorInsertmTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBL119-2
Plasmid#104336PurposeExpresses thsR from a TetR regulated promoter under ATC control. Expresses sfGFP from a thsR regulated promoter. Expresses mCherry from a constituative promoter.DepositorInsertThiosulfate response regulator
UseTagsExpressionBacterialMutationPromoterPLTetO1Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-SEIPIN3
Plasmid#96978PurposeExpress GFP-tagged Arabidopsis SEIPIN3 gene in plants.DepositorInsertSEIPIN3 (AT2G34380 Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-LDR
Plasmid#173857PurposeEncoding a part of the rapamycin-induced dimerization system.DepositorInserta Lyn11-targeted FRB (LDR)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
roGFP_iL_pQE30
Plasmid#48633PurposeBacterial expression vector encoding for the 6XHis tagged redox probe roGFPiL (roGFP C147, L147b, C204)DepositorInsertroGFP1
UseTagsHisTagExpressionMutationinsertion L147BPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-sgMDH1
Plasmid#72875PurposeThe lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEBDepositorInsertsgMDH1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D1 C7G HA
Plasmid#8954DepositorInsertcyclin D1 (CCND1 Human)
UseTagsHAExpressionMammalianMutationC7G: point mutation in LxCxE motif, LxghEPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pmiReport PU.1 3' UTR
Plasmid#26530DepositorInsertPU.1 3' UTR (Spi1 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-SEIPIN2
Plasmid#96977PurposeExpress GFP-tagged Arabidopsis SEIPIN2 gene in plants.DepositorInsertSEIPIN2 (AT1G29760 Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-SEIPIN1
Plasmid#96976PurposeExpress GFP-tagged Arabidopsis SEIPIN1 gene in plants.DepositorInsertSEIPIN1 (AT5G16460 Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D1 LxCxE HA
Plasmid#8956DepositorInsertcyclin D1 (CCND1 Human)
UseTagsHAExpressionMammalianMutationDeletion of LxCxE motifPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBW216J106-Amp32C
Plasmid#78651PurposepSB3K3 carrying J106-30hrpR-32hrpS-t-hrpL-30gfp-tTDepositorInsertJ106-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN1
Plasmid#96979PurposeExpress GFP-tagged Arabidopsis SEIPIN1 gene in plants.DepositorInsertSEIPIN1 (AT5G16460 Mustard Weed)
UseTags6xHis and GFPExpressionPlantMutationPromoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMH-HT_ygiF-FL
Plasmid#127795PurposeExpresses full-length ygiF in E. coli cellsDepositorInsertygiF (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL-Lats2UTR
Plasmid#20882DepositorInsertLats2 3'UTR (Lats2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBW214J115-Amp32C
Plasmid#78649PurposepSB3K3 carrying J115-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertJ115-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-taiman
Plasmid#17584DepositorInserttaiman (tai Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
As5 (pBW414J105-arsR)
Plasmid#123162PurposeArsenic sensor used in the sensor cell array. pSB3K3 carrying J105-30arsR-t-ParsR-30gfp-tDepositorInsertJ105-30arsR-t-ParsR-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ105, ParsRAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only