165,786 results

1. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

   Plasmid

   #172604

   Purpose

   Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_Pro-IL-1α

   Plasmid

   #214341

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL1A (IL1A Human)
   

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKD-SepRS-EFSep

   Plasmid

   #34623

   Depositor

   Jesse Rinehart, Dieter Söll

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Inserts

   SepRS (MMP0688 Methanocaldococcuc maripaludis)
   EF-Sep (tufB E. coli)
   

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Promoter

   Ptac and Ptac (from SepRS- operon like)

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-Puro-P2A-EGFP_mRFPstuf

   Plasmid

   #137730

   Purpose

   Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBIG2ab

   Plasmid

   #80616

   Purpose

   pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T-1 3xHA-MEK1-DD

   Plasmid

   #47576

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MEK1-DD (Map2k1 Mouse)
   

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Ser218 to Asp, Ser221 to Asp

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pMB-SaCas9

   Plasmid

   #105998

   Purpose

   SaCas9 expression vector

   Depositor

   Mike McManus

   Article

   Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

   Insert

   Cas9 nuclease
   

   Use

   CRISPR and Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   short EF1alpha

   Available Since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6_rd6_pegRNA_(PP7-C4-Q1)

   Plasmid

   #232433

   Purpose

   pegRNA with optimized 3' modifications to correct the rd6 mutation

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3-N90-beta-catenin

   Plasmid

   #31785

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of aa 1-90- Constitutitvely Active

   Promoter

   EF1alpha

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-NES-jRGECO1a

    Plasmid

    #61563

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRGECO1a
    

    Tags

    nuclear export signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. gag/pol

    Plasmid

    #14887

    Depositor

    Tannishtha Reya

    Article

    Reya et al Nature. 2003 May 22. 423(6938):409-14.

    Insert

    gag / pol
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. LifeAct-LSSmGFP

    Plasmid

    #186534

    Purpose

    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBtet-RN

    Plasmid

    #60503

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gi)

    Plasmid

    #230019

    Purpose

    AAV vector expressing anti-mCherry PAGER(Gi) in neurons

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM1D
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-hEAAT1

    Plasmid

    #32813

    Depositor

    Susan Amara

    Insert

    EAAT1 (SLC1A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV_Zika_NS3_Flag

    Plasmid

    #79635

    Purpose

    Third generation Self-inactivating lentivector expressing NS3 from Zika virus

    Depositor

    Vaithi Arumugaswami

    Insert

    NS3
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. RXFP1-Tango

    Plasmid

    #66492

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    RXFP1 (RXFP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pTRE3G-GSX2

    Plasmid

    #96964

    Purpose

    Dox-inducible expression of Gsx2 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GSX2 (Gsx2 Mouse)
    

    Expression

    Mammalian

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a_SOX10_P2A_Hygro_Barcode

    Plasmid

    #120479

    Purpose

    Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TroponinT-GCaMP5-Zeo

    Plasmid

    #46027

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    GCaMP5-2A-Zeo
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TNNT2

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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