9,002 results

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pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

Plasmid

#163047

Purpose

GCaMPer calcium sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMPer

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Promoter

None

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP657

Plasmid

#57824

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP657

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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SpyDock

Plasmid

#124618

Purpose

Expresses SpyDock to bind SpyTag non-covalently for affinity purification

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyDock

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iGluSnFR3.v857.GPI

Plasmid

#178335

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.GPI

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

GPI Anchor, IgK-chain, and Myc epi tag

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-MMLV_RT_D524N-6H

Plasmid

#166945

Purpose

Bacterial expression of M-MLV reverse transcriptase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

gag-pol

Tags

6X His

Expression

Bacterial

Mutation

D524N, H8Y

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGFPGUSPlus

Plasmid

#64401

Purpose

Binary plant vector expressing GFP and GUS, both driven by a 35S promoter

Depositor

Claudia Vickers

Article

Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

Inserts

GFP
GUSPlus

Use

Binary vector for plant transformation

Tags

His

Expression

Plant

Mutation

S65T

Promoter

35S

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pIRESneo-PA-GFP-alpha Tubulin

Plasmid

#12297

Depositor

Patricia Wadsworth

Article

Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

Insert

alpha Tubulin (TUBA1B Human)

Tags

PA-GFP

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-GFP-PGK-Puro

Plasmid

#110869

Purpose

Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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CHMP2A_GFP_N_term

Plasmid

#31805

Depositor

Daniel Gerlich

Article

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP2A (CHMP2A Human)

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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p3xnls-mScarlet3_N1

Plasmid

#189775

Purpose

Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

3xnls-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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T7-VEE-OKS-iG

Plasmid

#58974

Purpose

RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Inserts

Oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
GLIS (GLIS1 Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-coilin

Plasmid

#36906

Depositor

Greg Matera

Article

Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

Insert

coilin (COIL Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pMKO.1 GFP

Plasmid

#10676

Purpose

Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

Depositor

William Hahn

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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GFP-EEA1 wt

Plasmid

#42307

Depositor

Silvia Corvera

Article

Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

Insert

EEA1 (EEA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pMV306hsp+FFluc

Plasmid

#26156

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Firefly luciferase

Expression

Bacterial

Mutation

Firefly luciferase gene codon optimised for mycob…

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

Plasmid

#167582

Purpose

Express the ATP sensor GRAB_ATP1.0 in mammalian cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT WT

Plasmid

#84924

Purpose

luciferase reporter for TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

TERT

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SypHluorin 4x (S4x)

Plasmid

#37005

Depositor

Stephen Heinemann, Yongling Zhu

Article

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin 4x (Syp Rat)

Tags

HA

Expression

Mammalian

Mutation

Four copies of pHluorin inserted at site 184-185

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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