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pULTRA-CNF

Plasmid

#48215

Purpose

highly efficient suppressor plasmid

Depositor

Peter Schultz

Article

Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

Inserts

polyspecific MJ tyrosyl synthetase
tRNA

Expression

Bacterial

Mutation

Y32L, L65V, F108W, Q109M, D158G, I159A

Promoter

proK and tacI

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast

Plasmid

#125133

Purpose

Lentiviral empty backbone control vector with CMV promoter and Blasticidin resistance gene

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Type

Empty backbone

Use

Lentiviral

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-hSF3B1-K700E

Plasmid

#82577

Purpose

Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-K700E (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Mutation

K700E

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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hFerritinH

Plasmid

#122652

Purpose

Expression of hFerritinH in bacterial cells

Depositor

Trevor Douglas

Insert

ferritin heavy chain 1 (FTH1 Human)

Expression

Bacterial

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC2

Plasmid

#22975

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC2 (CRTC2 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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beta-arrestin2 mYFP

Plasmid

#36917

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin2 (Arrb2 Rat)

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV CreERT2 puro

Plasmid

#22776

Purpose

Retroviral expression of tamoxifen-inducible Cre-ERT2

Depositor

Tyler Jacks

Article

Kumar et al Genes Dev. 2009 Dec 1;23(23):2700-4. doi

Insert

CreERT2

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9

Plasmid

#47106

Purpose

Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Tags

Flag and SV40 NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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3541 pMIG Bcl-XL

Plasmid

#8790

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-XL (BCL2L1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymNeongreen-SpHis5

Plasmid

#125704

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Tags

ymNeonGreen

Expression

Yeast

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC1 T18D, S19D

Plasmid

#35682

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18D and S19D

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pBJ5 hBRG1

Plasmid

#17873

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg (SMARCA4 Human)

Expression

Mammalian

Mutation

R1608Q

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pTXB1-nbMmKappa-Tn5

Plasmid

#184286

Purpose

Anti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

New York Genome Center, Ivan Raimondi

Article

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbMmKappa-Tn5

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1(R40L)-GFP

Plasmid

#51408

Purpose

Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 40 mutated to leucine

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
TetON-shYAP

Plasmid

#115667

Purpose

Inducible YAP knockdown

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP shRNA (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI3M-TBD

Plasmid

#113077

Purpose

Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI3M-TBD

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159816

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET-19b_MMLV-RT

Plasmid

#153312

Purpose

Bacterial expression of MMLV reverse transcriptase

Depositor

Drew Endy, Philippa Marrack

Insert

MMLV Reverse Transcriptase

Tags

10x His

Expression

Bacterial

Available Since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-mNeptune2.5

Plasmid

#51310

Purpose

Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2.5

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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