We narrowed to 144,836 results for: ins

1. pRF0G-IodoY

   Plasmid

   #113645

   Purpose

   Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   IodoYRS and tRNA gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLVX-RT005-HaloGPM

   Plasmid

   #163385

   Purpose

   2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_human CDK1

   Plasmid

   #118597

   Purpose

   One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   guide RNA against human CDK1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBad-mKalama1

   Plasmid

   #14892

   Purpose

   Bacterial expression of mKalama1 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   mKalama1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

   Plasmid

   #232800

   Purpose

   Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

   Depositor

   Chunliang Li

   Article

   Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

   Insert

   OsTIR1 F74G EGFP-AID
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PKG I alpha WT

   Plasmid

   #16390

   Depositor

   Bernard Weinstein

   Article

   Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

   Insert

   PKG1 alpha (PRKG1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS59

   Plasmid

   #215680

   Purpose

   Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pP3-GFP

   Plasmid

   #53436

   Purpose

   Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   P3 promoter
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   P3

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORE303N

   Plasmid

   #194438

   Purpose

   CRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI site for gRNA insertion

   Depositor

   Chung-Jui Tsai

   Article

   Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28sumo-hRig-I DCARDS

    Plasmid

    #210828

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

    Insert

    hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SpyCatcher002-oDi

    Plasmid

    #124661

    Purpose

    Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oDi
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mNeonGreen-2A-mTurquoise2

    Plasmid

    #98885

    Purpose

    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mNeonGreen-2A-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PT7-deGFP-MGapt

    Plasmid

    #67741

    Purpose

    T7 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T7term
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUGW-hGtACR2-EYFP

    Plasmid

    #67877

    Purpose

    Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

    Depositor

    John Spudich

    Article

    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

    Insert

    hGtACR2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_Tiam1-pMag(3x)-iRFP

    Plasmid

    #67305

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    Tiam1-pMag(3x)-iRFP (Tiam1 Synthetic, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFUGW-hGtACR1-EYFP

    Plasmid

    #67795

    Purpose

    Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

    Depositor

    John Spudich

    Article

    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

    Insert

    hGtACR1
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRVL-2

    Plasmid

    #104580

    Purpose

    Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Mouse IgG2c CH1/CH2/CH3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SpyCatcher002-oTet

    Plasmid

    #124663

    Purpose

    Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oTet
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTM1_VP35_ZEBOV

    Plasmid

    #68121

    Purpose

    Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    VP35 ZEBOV (VP35 )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-NBid-PhoCl2c-CBid

    Plasmid

    #164051

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl2c

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl2c-CBid
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SpyCatcher002-oTri

    Plasmid

    #124662

    Purpose

    Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oTri
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTM1_VP30_ZEBOV

    Plasmid

    #69119

    Purpose

    Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    VP30 ZEBOV (VP30 )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDOC-K-glmS-GFPfor

    Plasmid

    #158059

    Purpose

    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    Green Fluorescent Protein optimised for excitation with UV light
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    acpP

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVX-RT001-LVB

    Plasmid

    #163381

    Purpose

    Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEEGFRtm-BFP (LVB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKLBac-FluB-trimer

    Plasmid

    #229859

    Purpose

    Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

    Inserts

    PA, Influenza B (B/Memphis/13/03)
    PB1, Influenza B (B/Memphis/13/03)
    PB2, Influenza B (B/Los Angeles/1/02)
    

    Use

    Tags

    2x-Streptag and 8xHis-tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-RT002-LV

    Plasmid

    #163382

    Purpose

    2nd generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEGFRtm (LV)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 3xF-GAR1

    Plasmid

    #126873

    Purpose

    Expresses 3xFlag GAR1

    Depositor

    Kathleen Collins

    Article

    Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

    Insert

    GAR1 (GAR1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS-PesaRlux

    Plasmid

    #47640

    Purpose

    wt esaR promoter in pCS26

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    PesaR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PesaR

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PKG I beta WT

    Plasmid

    #16394

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 beta (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge