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pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

Plasmid

#140203

Purpose

Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

crRNA array elcA

Use

CRISPR and Synthetic Biology

Promoter

PgpdA

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ:mTREK-1_cryst

Plasmid

#133269

Purpose

P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLCHKO_TK1_HPRT1_Lb_4

Plasmid

#155062

Purpose

Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF1CACTA1_3xFLAG_TET1cd

Plasmid

#106434

Purpose

pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

TET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)

Use

Synthetic Biology

Tags

3xFLAG

Expression

Plant

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJJR50

Plasmid

#75026

Purpose

U6 promoter driven flipped + extended sgRNA expression vector

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

guide RNA, flipped and extended version

Use

CRISPR

Promoter

R07E5.16 (U6)

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_TET1cd

Plasmid

#106432

Purpose

pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

pUBQ10::ZF108_3xFLAG_TET1cd (TET1 Mustard Weed, Synthetic, Human)

Use

Synthetic Biology

Expression

Plant

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHalo2X-mMYO7A-ΔSH3-ΔM/F2

Plasmid

#251427

Purpose

Mammalian expression vector for expressing HaloTag-fused mouse MYO7A truncated after the first MyTH4-FERM domain

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin VIIa (SH3 and M/F2 trancated) (Myo7a Mouse)

Tags

HaloTag

Expression

Mammalian

Mutation

The SH3 and M/F2 domains trancated

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-CaBLAM

Plasmid

#244129

Purpose

Bioluminescent calcium indicator

Depositor

Nathan Shaner

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Insert

CaBLAM

Expression

Mammalian

Mutation

Calmodulin N98I

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_RPP29 (pAVA3545)

Plasmid

#239314

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP29

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting RPP29 (POP4 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_POP5 (pAVA3498)

Plasmid

#239315

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting POP5

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting POP5 (POP5 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_RPP14 (pAVA3507)

Plasmid

#239317

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP14

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting RPP14 (RPP14 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_DDX59 (pAVA3791)

Plasmid

#239297

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting DDX59 (DDX59 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_DDX59 (pAVA3317)

Plasmid

#239299

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting DDX59 (DDX59 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_DDX59 (pAVA3794)

Plasmid

#239300

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting DDX59 (DDX59 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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sgYAP-R-3

Plasmid

#229447

Purpose

knockout of YAP1

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-R-3 (YAP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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sgMARK2-3-2

Plasmid

#229441

Purpose

knockout of MARK2 and MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK2-3-2 (MARK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8ΔChromo

Plasmid

#224708

Purpose

Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 chromodomain truncation (CBX8 Human)

Tags

HMBP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsRUNX

Plasmid

#205639

Purpose

reRNA guide targeting Homo sapiens RUNX

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsRUNX (RUNX1 Human)

Expression

Mammalian

Promoter

U6

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper Retro GFP DGK alfa human

Plasmid

#224578

Purpose

To stably downregulate the expression of human DGK alfa in human cells

Depositor

Isabel Mérida

Article

Torres-Ayuso et al Oncotarget. 2014 Oct 30;5(20):9710-26. d

Insert

sh RNAi Diacylglycerol kinase alfa human (DGKA Human)

Use

RNAi and Retroviral

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1295-AAV-EFSNC-dCjCas9-HP1a(72-177)

Plasmid

#223140

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 72-177

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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