We narrowed to 18,356 results for: Tec

1. IVT-MCP-MMLV-RT

   Plasmid

   #207457

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   MCP-RT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24_NusA-Tus

   Plasmid

   #165959

   Purpose

   Expression of soluble E. coli Tus protein

   Depositor

   Keith Pardee

   Article

   Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

   Insert

   Tus
   

   Use

   Tags

   His-NusA-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. apt-CBE-3'end

   Plasmid

   #219939

   Purpose

   Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   MCP_32-aa_evo-rA1_2xUGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. apt-ABE8e

   Plasmid

   #219937

   Purpose

   Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Com_93-aa_TadA8e
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 TCF12

   Plasmid

   #67113

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TCF12 (TCF12 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pVH233-Tier1-PmPGK1-eYFP

   Plasmid

   #169522

   Purpose

   Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a.Tat(48-57)-I-SceI

   Plasmid

   #165041

   Purpose

   Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   I-SceI
   

   Use

   Tags

   6XHis and Tat(48-57)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBLO1806_Cas9_2xNLS_human

   Plasmid

   #74493

   Purpose

   Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   Cas9
   

   Use

   Tags

   t2a mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19/Vc-C

   Plasmid

   #183158

   Purpose

   Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PE-Cas9

    Plasmid

    #174851

    Purpose

    Express PE-Cas9 in mammalian cells

    Depositor

    Wen Xue

    Article

    Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    PE-Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGNW4

    Plasmid

    #122088

    Purpose

    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK295

    Plasmid

    #59564

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) 2xK-turn Kozak EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Neq2X

    Plasmid

    #182365

    Purpose

    Expresses Neq2X DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTK095

    Plasmid

    #59560

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTW195b63

    Plasmid

    #136931

    Purpose

    Directed evolution of SpCas11

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII(N)-npuN
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCIDT-AMP-Bv16SrRNA

    Plasmid

    #218518

    Purpose

    pUCIDT-AMP containing 16S rRNA gene of B. vulgatus for absolute concentration quantification

    Depositor

    Ami Bhatt

    Article

    Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

    Insert

    16S rRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSBY3_ligD

    Plasmid

    #104624

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTK296

    Plasmid

    #59565

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) 2xK-turn Kozak EGFP-PEST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTol2-hspDRv7_scGstlt

    Plasmid

    #108870

    Purpose

    scGESTALT V7 lineage barcode for zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Insert

    scGESTALT lineage tracing target V7
    

    Use

    Tol2 integration into zebrafish genome

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-L1-PGK-TKNeo-L2

    Plasmid

    #141008

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    TKNeo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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