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Showing: 7701 - 7750 of 167150 results

  1. pGM1192

    Plasmid
    #115683
    Purpose
    E. coli-Streptomyces shuttle vector for gene expression
    Depositor
    Günther Muth
    Insert
    mCherry, aac(3)IV, tsr
    Tags
    Strep-tagII on mCherry, codon optimized
    Expression
    Bacterial
    Mutation
    mCherry codon optimized for Streptomyces
    Promoter
    SF14*
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    115683-plasmid-map-sequence-id-237128

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  2. pDual_dsCas9_BFP

    Plasmid
    #214679
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214679-plasmid-map-sequence-id-428808

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  3. mU6-sgSik1_2nd-hU6-sgSik3_2nd

    Plasmid
    #177219
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_2nd/sgSik3_2nd
    Use
    Lentiviral
    Promoter
    mU6/hU6
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177219-plasmid-map-sequence-id-350035

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  4. pCW57.1_TStrp_HA_Ct

    Plasmid
    #194066
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Twin_Strep-HA
    Expression
    Mammalian
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194066-plasmid-map-sequence-id-381940

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  5. pZP06B

    Plasmid
    #232321
    Purpose
    Make sacB a counterselection marker to replace galK
    Depositor
    Chenggang Wu
    Insert
    PrpsJ-sacB
    Expression
    Bacterial
    Available Since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232321-plasmid-map-sequence-id-462085

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  6. pX2-Cas9

    Plasmid
    #85811
    Purpose
    Arabinose inducible Cas9 in a broad host range backbone.
    Depositor
    Ryan Gill
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    85811-plasmid-map-sequence-id-177180

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  7. pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

    Plasmid
    #128174
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128174-plasmid-map-sequence-id-247966

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  8. pEC1.2/EC1.1_spCas9_TagRFP

    Plasmid
    #161934
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    161934-plasmid-map-sequence-id-320739

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  10. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. LT3GEPIR-WEE1shRNA1

    Plasmid
    #220571
    Purpose
    To inducibly knockdown WEE1 expression
    Depositor
    Sean Lee
    Insert
    WEE1 shRNA1 (WEE1 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220571-plasmid-map-sequence-id-438397

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  12. LT3GEPIR-WEE1shRNA2

    Plasmid
    #220572
    Purpose
    To inducibly knockdown WEE1 expression
    Depositor
    Sean Lee
    Insert
    WEE1 shRNA2 (WEE1 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220572-plasmid-map-sequence-id-437939

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  13. TRIP8b (1b-2)-GFP/pEGFP-C1

    Plasmid
    #197336
    Purpose
    Plasmid expressing an antigen targeting TRIP8b (exon 1b)
    Depositor
    Steve Siegelbaum
    Insert
    TRIP8b (1b-2) (Pex5l Mouse)
    Tags
    eGFP (GFP observed)
    Expression
    Mammalian
    Available Since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    197336-plasmid-map-sequence-id-401590

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  14. pcDNA3.1-hRIPK1-Flag

    Plasmid
    #112487
    Purpose
    Mammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112487-plasmid-map-sequence-id-214532

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  15. mu-GM-CSF-pCIneo

    Plasmid
    #163518
    Purpose
    Expresses murine GM-CSF in mammalian cells
    Depositor
    Thomas Weber
    Insert
    murine GM-CSF
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163518-plasmid-map-sequence-id-321579

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  16. pcDNA3.1 ShhN

    Plasmid
    #37680
    Depositor
    Philip Beachy
    Insert
    shh (Shh Mouse)
    Expression
    Mammalian
    Mutation
    soluble form (see comments)
    Promoter
    CMV
    Available Since
    July 10, 2012
    Availability
    Academic Institutions and Nonprofits only
    37680-plasmid-map-sequence-id-159475

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  17. R777-E221 Hs.RALGDS

    Plasmid
    #70505
    Purpose
    Gateway ORF clone of human RALGDS [NM_006266.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RALGDS (RALGDS Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70505-plasmid-map-sequence-id-125954

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  18. p15TV-L

    Plasmid
    #26093
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Sept. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    26093-plasmid-map-sequence-id-52817

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  19. pLKO-puro-hPPM1A

    Plasmid
    #185382
    Purpose
    For mammalian expression of shRNA: AGGGTAATGGGTTGCGATATG that targets human PPM1A
    Depositor
    Ayaz Najafov
    Insert
    PPM1A (PPM1A Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185382-plasmid-map-sequence-id-363721

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  21. pLenti-hSyn1-eNpHR3.0-iRFP713

    Plasmid
    #197594
    Purpose
    Lentiviral transfer vector for the neuronal expression of eNpHR3.0-iRFP713 (halorhodopsin for the inhibition of neuronal activity)
    Depositor
    Patrik Verstreken
    Insert
    eNpHR3.0-iRFP713
    Use
    Lentiviral
    Promoter
    hSynI
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197594-plasmid-map-sequence-id-389166

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  22. mEmerald-TPT1-C-10

    Plasmid
    #54283
    Purpose
    Localization: Microtubule Associated Protein, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    TPT1 (TPT1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. pIVTR(T7A)-NGN2 (SA Substitutions)

    Plasmid
    #172308
    Purpose
    In vitro transcription of NGN2 (Ser phosphosites substituted by Ala)
    Depositor
    Patrik Verstreken
    Insert
    NGN2
    Use
    Other
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A an…
    Promoter
    T7 promoter A-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172308-plasmid-map-sequence-id-340455

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  24. R777-E245 Hs.RASGRP2

    Plasmid
    #70529
    Purpose
    Gateway ORF clone of human RASGRP2 [NM_153819.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASGRP2 (RASGRP2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70529-plasmid-map-sequence-id-125978

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  25. pLenti CMVtight eGFP Blast (w782-1)

    Plasmid
    #26584
    Purpose
    2nd generation lentiviral vector
    Depositor
    Eric Campeau
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Lentiviral; Tet-on advanced vector
    Expression
    Mammalian
    Available Since
    Jan. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26584-plasmid-map-sequence-id-12594

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  26. pBAD_dGFPmut3-AAV

    Plasmid
    #187379
    Purpose
    arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187379-plasmid-map-sequence-id-370386

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  27. Lenti puro EF2 LUC-2A-Dendra2

    Plasmid
    #129427
    Purpose
    expression of LUC and Dendra2 reporters under EF2 promoter
    Depositor
    Oskar Laur
    Insert
    LUC Dendra2
    Use
    Lentiviral
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129427-plasmid-map-sequence-id-255690

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  28. pOpen-THEAQpolA

    Plasmid
    #165512
    Purpose
    DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Taq DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165512-plasmid-map-sequence-id-339400

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  29. pCSIIbleo-iRFP713(V256C)-P2A-EGFP

    Plasmid
    #198061
    Purpose
    The lentiviral vector for iRFP713(V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(V256C)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    V256C mutation
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198061-plasmid-map-sequence-id-389836

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  30. pDual_dsCas9_Venus

    Plasmid
    #214678
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214678-plasmid-map-sequence-id-428813

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  32. mPA-GFP-N1

    Plasmid
    #54712
    Purpose
    Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mPA-GFP
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pAdx-CMV-iCre-P2A-tdTomato

    Plasmid
    #73351
    Purpose
    Express iCre and tdTomato under CMV promoter
    Depositor
    Kazuhiro Oka
    Insert
    CMV-iCre-tdtomato
    Use
    Adenoviral
    Promoter
    CMV
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73351-plasmid-map-sequence-id-135084

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  36. pAAV.synP.DIO.EGFP.WPRE.hGH

    Plasmid
    #100043
    Purpose
    An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).
    Depositor
    Ian Wickersham
    Has Service
    AAV9
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    synapsin-1
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100043-plasmid-map-sequence-id-193613

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  37. pLV_Zika_NS2B_Flag

    Plasmid
    #79637
    Purpose
    Third generation Self-inactivating lentivector expressing NS2B from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS2B
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79637-plasmid-map-sequence-id-151178

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  38. pJA5 H-2K(b)

    Plasmid
    #180441
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2K(b)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    180441-plasmid-map-sequence-id-355754

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  39. 1056 pSG5L HA PTEN 380-385E

    Plasmid
    #11171
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    E4: S380E, T382E, T383E, S385E
    Available Since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  40. MSCV-Six1

    Plasmid
    #49263
    Purpose
    retroviral expression of human Six1 and GFP
    Depositor
    Heide Ford
    Insert
    Six1 (SIX1 Human)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Available Since
    Nov. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49263-plasmid-map-sequence-id-152187

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  41. pOpen-T7gene5

    Plasmid
    #165534
    Purpose
    DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T7 DNA Polymerase (unmodified)
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165534-plasmid-map-sequence-id-339124

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  42. pDEST-Spe4-RfA-mScarlet-I-stop

    Plasmid
    #186393
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    186393-plasmid-map-sequence-id-373587

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  43. pSLfa[UAS-Tc'hsp_p-tGFP-SV40]fa

    Plasmid
    #86452
    Purpose
    UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Shuttle plasmid); tGFP can be exchanged by other ORFs
    Depositor
    Gregor Bucher
    Insert
    UAS-Tc'hsp_p-tGFP-SV40
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86452-plasmid-map-sequence-id-170902

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  44. pLV_Zika_NS4B_Flag

    Plasmid
    #79640
    Purpose
    Third generation Self-inactivating lentivector expressing NS4B from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS4B
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79640-plasmid-map-sequence-id-148808

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  45. pCLXSN(GFP)-hp100

    Plasmid
    #174734
    Purpose
    Gene overexpression
    Depositor
    Shao-Cong Sun
    Insert
    P100 (NFKB2 Human)
    Use
    Retroviral
    Mutation
    WT
    Available Since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174734-plasmid-map-sequence-id-343213

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  47. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215011
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Available Since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215011-plasmid-map-sequence-id-429274

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  49. pLVU/myc

    Plasmid
    #24179
    Depositor
    Lars Ittner
    Insert
    Chloramphenicol resistance gene (CmR) - ccdB gene
    Use
    Lentiviral; Gateway
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Nov. 29, 2010
    Availability
    Academic Institutions and Nonprofits only

  50. pAG GST Empty

    Plasmid
    #236735
    Purpose
    Empty bacterial expression plasmid for GST fusion proteins
    Depositor
    Melina Fan, Addgene Research Program
    Type
    Empty backbone
    Tags
    GST
    Expression
    Bacterial
    Available Since
    April 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236735-plasmid-map-sequence-id-469201

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Showing: 7701 - 7750 of 167150 results