We narrowed to 18,363 results for: tec

1. pBLO1808_arC9_2xNLS_human

   Plasmid

   #74491

   Purpose

   Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   arC9
   

   Use

   Tags

   t2a mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

   Plasmid

   #55216

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBT373

   Plasmid

   #125614

   Purpose

   Expresses FERNY-BE4max-NG in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   FERNY-BE4max-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. E.coli RaPID plasmid

   Plasmid

   #107251

   Purpose

   Expresses the E.coli RaPID labeling protein

   Depositor

   Paul Khavari

   Article

   Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

   Insert

   E.coli BirA*
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24_NusA-Tus

   Plasmid

   #165959

   Purpose

   Expression of soluble E. coli Tus protein

   Depositor

   Keith Pardee

   Article

   Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

   Insert

   Tus
   

   Use

   Tags

   His-NusA-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. IVT-MCP-MMLV-RT

   Plasmid

   #207457

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   MCP-RT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. apt-CBE-3'end

   Plasmid

   #219939

   Purpose

   Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   MCP_32-aa_evo-rA1_2xUGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. apt-ABE8e

   Plasmid

   #219937

   Purpose

   Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Com_93-aa_TadA8e
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 TCF12

   Plasmid

   #67113

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TCF12 (TCF12 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pVH233-Tier1-PmPGK1-eYFP

    Plasmid

    #169522

    Purpose

    Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBLO1806_Cas9_2xNLS_human

    Plasmid

    #74493

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a.Tat(48-57)-I-SceI

    Plasmid

    #165041

    Purpose

    Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    I-SceI
    

    Use

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PE-Cas9

    Plasmid

    #174851

    Purpose

    Express PE-Cas9 in mammalian cells

    Depositor

    Wen Xue

    Article

    Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    PE-Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19/Vc-C

    Plasmid

    #183158

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTK095

    Plasmid

    #59560

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTK295

    Plasmid

    #59564

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) 2xK-turn Kozak EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-Neq2X

    Plasmid

    #182365

    Purpose

    Expresses Neq2X DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTW195b63

    Plasmid

    #136931

    Purpose

    Directed evolution of SpCas11

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII(N)-npuN
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBY3_ligD

    Plasmid

    #104624

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTol2-hspDRv7_scGstlt

    Plasmid

    #108870

    Purpose

    scGESTALT V7 lineage barcode for zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Insert

    scGESTALT lineage tracing target V7
    

    Use

    Tol2 integration into zebrafish genome

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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