We narrowed to 7,127 results for: trac

1. Pdg459-eSp(1.1) V3

   Plasmid

   #226965

   Purpose

   CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DG SaCas9 puro V3

   Plasmid

   #226960

   Purpose

   CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PDG458 eSp(1.1) V3

   Plasmid

   #226964

   Purpose

   CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPRKCZ-C20-EGFP (C-PRKCZ-C20)

   Plasmid

   #217761

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C20 domain (aa 405-592)

   Promoter

   CMV

   Available since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

   Plasmid

   #217763

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

   Promoter

   CMV

   Available since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

   Plasmid

   #217762

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Linker GGSSGGGA plus C20 domain (aa 405-592)

   Promoter

   CMV

   Available since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. AAV-Dlx-SynaptoTAG

   Plasmid

   #210729

   Purpose

   AAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.

   Depositor

   Wei Xu

   Article

   Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

   Insert

   EGFP-Synaptobrevin-2, P2A, Palm-tdTomato (tdTomato with Palmitoylation sequence) (Vamp2 Synthetic, Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Dlx

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUb FLAG-Zebrafish IntS6L

   Plasmid

   #198411

   Purpose

   Expresses FLAG-tagged zebrafish IntS6L

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   ints6l (ints6l Zebrafish)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ubi-p63e

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUb FLAG-Zebrafish IntS6

   Plasmid

   #198410

   Purpose

   Expresses FLAG-tagged zebrafish IntS6

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   ints6 (ints6 Zebrafish)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ubi-p63e

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Hy_U5:34A eGFP SV40

    Plasmid

    #195064

    Purpose

    Expresses eGFP when there is readthrough transcription from the Drosophila U5:34A gene

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    snRNA:U5:34A (snRNA:U5:34A Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U5:34A

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUb FLAG-cka

    Plasmid

    #195067

    Purpose

    Expresses FLAG-tagged Drosophila cka protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Connector of kinase to AP-1 (Cka Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUb FLAG-wdb

    Plasmid

    #195069

    Purpose

    Expresses FLAG-tagged Drosophila wdb protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    widerborst (wdb Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUb FLAG-wrd

    Plasmid

    #195070

    Purpose

    Expresses FLAG-tagged Drosophila wrd protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    well-rounded (wrd Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV_PGK_3xHA-LTB4R(T308A)

    Plasmid

    #208092

    Purpose

    Mammalian expression of the human LTB4 receptor LTB4R with the T308A mutation with an N-terminal 3x HA tag.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    3xHA-LTB4R(T308A) (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T308A

    Promoter

    PGK

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV_PGK_3xHA-LTB4R(S310A)

    Plasmid

    #208093

    Purpose

    Mammalian expression of the human LTB4 receptor LTB4R with the S310A mutation with an N-terminal 3x HA tag.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    3xHA-LTB4R(S310A) (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    S310A

    Promoter

    PGK

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV_PGK_3xHA-LTB4R(T308A,S310A)

    Plasmid

    #208094

    Purpose

    Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations with an N-terminal 3x HA tag.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    3xHA-LTB4R(T308A,S310A) (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T308A, S310A

    Promoter

    PGK

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD

    Plasmid

    #201818

    Purpose

    A plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoter

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    V5-ER-LBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tetO-IRX5

    Plasmid

    #170693

    Purpose

    doxycycline-inducible overexpression of IRX5

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    IRX5 (IRX5 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tetO-ZFPM2

    Plasmid

    #170707

    Purpose

    doxycycline-inducible overexpression of ZFPM2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    ZFPM2 (ZFPM2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    3 nonsynonymous changes: 353C>T (Ser>Phe)…

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQCXIH FRB-BFP

    Plasmid

    #202425

    Purpose

    TagBFP FKBP-rapamycin binding (FRB) dimerization domain

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    FKBP-rapamycin binding (FRB) dimerization domain
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge