We narrowed to 7,103 results for: GFP expression plasmids
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Plasmid#140245PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE_VRQR
ExpressionMammalianMutationV82G in TadA*, VRQR mutations in SpCas9, D10A in …PromoterCMVAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG302-SunTag-FWAgRNA-4-22aa-TET1cd
Plasmid#115344PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJP_1node
Plasmid#230984PurposepBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate mCitrine expression.DepositorInsertmCitrine
ExpressionBacterialAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ054 (PB)SFFV-LIDARsensor (hygR)
Plasmid#233419PurposePiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, EGFP as output) for stable integrationDepositorInsertmCherry-SGc(stem-1(3xMS2))cSG-EGFP
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGFAP-Halo
Plasmid#169474Purposethe host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sitesDepositorAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
SMT503 - S77A
Plasmid#226842PurposePlasmid that encodes a B. Subtilis lipase (CLIP-BsLipA-eGFP) gene fusion with the S77A catalytic mutant. Compatible with PURExpress for in vitro transcription/translation. ChlorampDepositorInsertLipase A
UseSynthetic BiologyTagsSNAP and eGFPExpressionBacterialMutationR33Q, D34N, K35D, S77A, K112D, M134D, Y139C, I157MPromoterT7Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
SMT503
Plasmid#226840PurposePlasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. ChlorampDepositorInsertLipase A
UseSynthetic BiologyTagsSNAP and eGFPExpressionBacterialMutationR33Q, D34N, K35D, K112D, M134D, Y139C, I157MPromoterT7Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN10042
Plasmid#89052PurposeGF-ori-FP plasmid for GFxFP assayDepositorInsertsEGFP part 1
EGFP part 2
UseCRISPRExpressionMammalianPromoterCAG and noneAvailable SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)
Plasmid#223111PurposepCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2DepositorInserthuman codon optimized nuclease SpCas9-P2A-mTagBFP2
UseCRISPRTagsBPNLS-3xFLAG-P2A-mTagBFP2ExpressionMammalianPromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-14 (LA-14)
Plasmid#158750PurposeLentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.DepositorInsertLifeAct-14
UseLentiviralTagseGFPExpressionMammalianMutationDeletion mutation for last three amino acids from…PromoterCMV immediate earlyAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ACTB CRISPIE
Plasmid#172853PurposeActb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.DepositorInsertsActb intron 1 sgRNA
DRS-2 sgRNA
CRISPIE designer exon (phase 0-0) encoding mEGFP
mRuby3
UseAAV and CRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-DNMT3a(R887E)-DNMT3l
Plasmid#154141PurposeExpresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
TagsHA and sfGFPExpressionMammalianMutationR887EPromoterSFFVAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniABEmax (pRZ900)
Plasmid#131311PurposeCMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).DepositorInsertbpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIC306
Plasmid#52257PurposeRetroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)DepositorInsertSka3 (SKA3 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaMK2rep-S603A
Plasmid#239623PurposeInactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.DepositorInsertSpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
TagsSpot, myc, and sGFP2ExpressionMammalianMutationchanged Serine 603 to AlaninePromoterCMVAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
SECURE miniABEmax-V82G (pJUL1828)
Plasmid#131313PurposeCMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).DepositorInsertbpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable SinceSept. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FHUG+W (B2)
Plasmid#74011Purposelentiviral expression of EGFP and shRNA (from H1 promoter, empty)DepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
NaChBac pTracer CMV2
Plasmid#60835PurposeMammalian expression plasmid containing NaChBac gene with an EGFP reporterDepositorInsertNaChBac
ExpressionMammalianPromoterCMVAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 2)
Plasmid#191342PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 2)
ExpressionMammalianPromoterTRE3GVAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 1)
Plasmid#191341PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 1)
ExpressionMammalianPromoterTRE3GVAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2
Plasmid#217635PurposeAAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.DepositorInsertmU6-sgRNA, Handle sequence, EF1a-mTagBFP2
UseAAVPromoterEF1alpha and mU6Available SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-U6-gTgfbr2-CBh-mCherry-SV40late
Plasmid#249125Purposeexpression of gTgfbr2 and mCherryDepositorAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-U6-gThy1-CBh-mCherry-SV40late
Plasmid#249126Purposeexpression of gThy1 and mCherryDepositorAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-U6-gTnfrfs1a-CBh-mCherry-SV40late
Plasmid#249127Purposeexpression of gTnfrsf1a and mCherryDepositorAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-KIKO-U6-gOsmr-p2a-mCherry-STOP-pA-400
Plasmid#249130Purposeall-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gOsmr cut site, disrupting Osmr expressionDepositorInsertgOsmr, mCherry (Osmr Mouse, Synthetic)
UseAAVAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-U6-gCtnnb1-CBh-mCherry-SV40late
Plasmid#249119Purposeexpression of gCtnnb1 and mCherryDepositorAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald
Plasmid#214469PurposeLentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable SinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
ρ1-EM GABAA receptor in pEZT-BM
Plasmid#213072PurposeExpress human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICDDepositorInsertHuman GABAa rho1 receptor (GABRR1 Human)
UseBacmam, baculovirus, oocyte expressionTagsTwin-Strep tag and superfolderGFPExpressionMammalianMutationEncodes residues S58–L383 and D451–S479, separate…PromoterCMV and T7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0248
Plasmid#162313PurposeReporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiTDepositorInsertT7prom-RBS-sfGFP-HiBiT
UseSynthetic BiologyAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0332
Plasmid#162314PurposeReporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiTDepositorInsertSP6prom-EO1-sfGFP-HiBiT
UseSynthetic BiologyAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMulti-sgRNA-LacZ
Plasmid#99913PurposeReceptor plasmid for the assembly of multiple sgRNAsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6.shRNA-Sdc1-1376
Plasmid#195574PurposeExpresses shRNA to knockdown mouse Syndecan-1DepositorAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dPlcR(K30/32L)
Plasmid#181833PurposePhospholipid binding-deficient Lyn-dPlcR negative-control plasmid.DepositorInsertLyn-dPlcR (K30L,K32L)
TagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationLysines 30 and 32 in PH domain mutated to leucine.PromoterCMVAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMulti-sgRNA-LacZ-DsRed
Plasmid#99914PurposeReceptor plasmid for the assembly of multiple sgRNAs with DsRed coexpressionDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
BNLS
Plasmid#50836PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).DepositorInsertddGFP B
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
sTR056
Plasmid#177369PurposeToehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2
Plasmid#216173PurposeExpress the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- FOXA.bs- ZEB2.locus
UseLentiviralTagsEGFPAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
SECURE miniABEmax-K20A/R21A (pJUL1774)
Plasmid#131312PurposeCMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).DepositorInsertbpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
hA3A-BE3 (pRZ215)
Plasmid#131314PurposeCAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.DepositorInserthA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
ExpressionMammalianPromoterCAGAvailable SinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
hAID-BE3 (pRZ352)
Plasmid#131316PurposeCAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.DepositorInserthAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
ExpressionMammalianPromoterCAGAvailable SinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only