We narrowed to 10,000 results for: ADA

1. NavBp

   Plasmid

   #100014

   Purpose

   Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

   Depositor

   Paul DeCaen, David Clapham

   Article

   DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

   Insert

   NavBp
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-ALFA-tag-Gal4-VP16

   Plasmid

   #199465

   Purpose

   Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

   Insert

   Gal4-ALFA-tag-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJ823

   Plasmid

   #39313

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9 (lin2744 Listeria innocua)
   

   Use

   CRISPR

   Tags

   MBP and thioredoxin-His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

4. mTurquoise2-PXN

   Plasmid

   #112957

   Purpose

   In vivo visualization of focal adhesions (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   PXN
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 S6K2 KR

   Plasmid

   #17730

   Depositor

   John Blenis

   Article

   Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

   Insert

   S6K2 (RPS6KB2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K99R: kinase dead mutant

   Promoter

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

6. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS415_pGal-nCas9(H840A)

   Plasmid

   #79616

   Purpose

   Expresses nCas9(H840A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H840A for nickase Cas9

   Promoter

   pGal1

   Available Since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-IREShygro2

   Plasmid

   #153966

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   EGFP
   

   Use

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CMV promoter

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-EF1a-mTagBFP-T2A-TetOn3G

   Plasmid

   #179888

   Purpose

   Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   TetOn3G
   

   Use

   Lentiviral

   Tags

   mTagBFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. EMTB-mTurquoise2

    Plasmid

    #137803

    Purpose

    Visualization of microtubules

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Ensconsin microtubule binding domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEBG-TRAF2 (GST)

    Plasmid

    #21586

    Depositor

    John Kyriakis

    Article

    Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

    Insert

    TNF receptor-associated factor 2 (TRAF2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

12. LRP6 deltaN-pCS2-VSVG

    Plasmid

    #27281

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Expression

    Mutation

    contains amino acid residues 1370–1613 (extracell…

    Promoter

    Available Since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. px458_2A_GFP_sgRNA_ZNF598

    Plasmid

    #127121

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA ZNF598 (ZNF598 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJW1354

    Plasmid

    #69490

    Purpose

    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

    Depositor

    Jordan Ward

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    

    Use

    CRISPR; Template to generate cassettes for -media…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1 Flag Raptor HRas25

    Plasmid

    #26637

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    raptor-HRas25 (RPTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CAG-mNeptune2.5-P2A-GFP

    Plasmid

    #197156

    Purpose

    Expresses the protein of mNeptune2.5-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    mNeptune2.5-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfuNca-Plastid

    Plasmid

    #190818

    Purpose

    Plastid targeting GFP with ble resistant gene for zeocin

    Depositor

    Peter Kroth

    Article

    Sprecher et al Algal Research. 2023 May; Vol 72: 103136

    Inserts

    Diatom plastid targeted GFP
    Bleomycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Cylindrotheca fusiformis fcp and Nitzschia captiv…

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Promoter

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Clathrin-LCa-ECFP

    Plasmid

    #21742

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

20. sABE v3.22 Lenti_2

    Plasmid

    #209556

    Purpose

    pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB

    Depositor

    Xue Gao

    Article

    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

    Insert

    SpCas9 sgRNA, TadA-8e (2-76)-2xFRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pU6, pCMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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