We narrowed to 10,838 results for: ENA

1. pcDNA3.1(+)/AUG-nLuc-3XFLAG

   Plasmid

   #127299

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDIRECT_21A

   Plasmid

   #91128

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLP712

   Plasmid

   #203741

   Purpose

   Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   OsTIR1
   

   Use

   Candida albicans integrating vector

   Tags

   3xMyc

   Expression

   Mutation

   Codon 74 changed from Phe to Ala (F74A)

   Promoter

   ACT1 (C. albicans)

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOD_B2520

   Plasmid

   #91077

   Purpose

   Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   OsU6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRH623

   Plasmid

   #171633

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-non-targeting
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC-nSyb-IVS-PhiC31

   Plasmid

   #133868

   Purpose

   Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   PhiC31 Recombinase (int )
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAT007

   Plasmid

   #171634

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. REEP6-EGFP

   Plasmid

   #210364

   Purpose

   Expresses REEP6 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   REEP6 (REEP6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FgH1tUTG_mup53_Ex4

   Plasmid

   #85534

   Purpose

   Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   mu p53
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   H1t

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-Venus-pLenti6

    Plasmid

    #36948

    Depositor

    William Kaelin

    Article

    Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

    Insert

    Venus (EYFP)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    -F46L/F64L/V68L/M153T/V163A/S175G

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_B2515

    Plasmid

    #91072

    Purpose

    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pREDIT_Cas9-MS2-BB_BbsI

    Plasmid

    #164802

    Purpose

    REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAC_C_PTH2

    Plasmid

    #111674

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    PTH2 (PTRH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe-FLAG-Neuroserpin

    Plasmid

    #58261

    Purpose

    Retroviral vector for expressing Neuroserpin in mammalian cells

    Depositor

    Joan Massague

    Article

    Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

    Insert

    Neuroserpin (SERPINI1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

16. Cas12j-8-puro

    Plasmid

    #194966

    Purpose

    The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

    Depositor

    Yongming Wang

    Article

    Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLyGo-Kp-1

    Plasmid

    #163143

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-sgArid1a-sg3

    Plasmid

    #192169

    Purpose

    Lenti-sgArid1a-sg3

    Depositor

    Sidi Chen

    Article

    Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pREDIT_Cas9n-MS2-BB_BbsI

    Plasmid

    #164804

    Purpose

    REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSET-WHaloCaMP1a-EGFP

    Plasmid

    #205303

    Purpose

    Expression of WHaloCaMP1a-EGFP fusion in E. coli

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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