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170,823 results

Showing: 6241 - 6280 of 170823 results

  8. pEf1a-hifiDn29-dCas9-P2A-GFP

    Plasmid
    #247158
    Purpose
    Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Insert
    hifiDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247158-plasmid-map-sequence-id-491688

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  11. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid
    #117699
    Purpose
    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
    Depositor
    Joerg Mansfeld
    Article
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    Tags
    HA tag, Myc-NES, and NLS
    Expression
    Mammalian
    Mutation
    codon optimized for murine expression
    Promoter
    None, 2A peptide from porcine teschovirus-1 polyp…
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    117699-plasmid-map-sequence-id-368109

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  12. HTR1B-DuET

    Plasmid
    #213317
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR1B (HTR1B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213317-plasmid-map-sequence-id-430854

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  16. pAAV-Ucp1-Cre-miR122

    Plasmid
    #228408
    Purpose
    Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.
    Depositor
    Joerg Heeren
    Article
    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1
    Inserts
    cre recombinase with SV40 NLS
    Cre recombinase with SV40 NLS
    Use
    AAV
    Promoter
    rat Ucp1 enhancer-promoter
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    228408-plasmid-map-sequence-id-459311

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  18. pGG-NES-TurboID-GFP

    Plasmid
    #209401
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    NES-3XHA-TurboID
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available Since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209401-plasmid-map-sequence-id-418454

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  22. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

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  23. pYPQ230-RRVQ

    Plasmid
    #200188
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRVQ
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V, E795Q
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    200188-plasmid-map-sequence-id-396764

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  24. TFORF1275

    Plasmid
    #143781
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TPRX1 (TPRX1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143781-plasmid-map-sequence-id-391569

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  35. pD649-HAsp-ISLR2_Ig1-3-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157619
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    ISLR2_Ig1-3 (ISLR2 Human)
    Tags
    Fc(DAPA)-Avi-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    157619-plasmid-map-sequence-id-382008

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  38. pEMS1600

    Plasmid
    #29274
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PN
    Depositor
    Elizabeth Simpson
    Article
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Insert
    Ple129 (MKI67 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available Since
    Nov. 4, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29274-plasmid-map-sequence-id-13689

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  40. Cas9 CFP

    Plasmid
    #155278
    Purpose
    Lentiviral Cas9 expression with CFP marker
    Depositor
    Martin Carroll
    Insert
    spCas9
    Expression
    Mammalian
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155278-plasmid-map-sequence-id-309981

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Showing: 6241 - 6280 of 170823 results