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Showing: 6241 - 6280 of 8366 results

  1. pAAV-CaMKIIa-hM3D(Gq)-mCherry

    Plasmid
    #50476
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50476-plasmid-map-sequence-id-171073

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  2. 2xMARS

    Plasmid
    #205233
    Purpose
    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    Use
    Tags
    Nuclear Export Sequence and mScarlet-i
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205233-plasmid-map-sequence-id-404744

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  3. pLV-Golgi eGFP

    Plasmid
    #79809
    Purpose
    To express and target GFP into the Golgi apparatus using lentiviruses
    Depositor
    Pantelis Tsoulfas
    Insert
    Golgi eGFP (B4GALT1 Human)
    Use
    Lentiviral
    Tags
    Fusion of the first 62 amino acids of the human b…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    79809-plasmid-map-sequence-id-150299

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  4. pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50472
    Purpose
    Gs-coupled rM3D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Bryan Roth
    Has Service
    AAV5
    Insert
    rM3D-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50472-plasmid-map-sequence-id-75495

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  5. EEF1A2 in pENTR

    Plasmid
    #216585
    Purpose
    gateway cloning donor vector containing EEF1A2
    Depositor
    Douglas Allan, Christopher Loewen
    Insert
    EEF1A2 (EEF1A2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216585-plasmid-map-sequence-id-426880

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  7. pJCX3-ACTG2_R257C

    Plasmid
    #228911
    Purpose
    Expresses mutant R257C smooth muscle actin gamma-2 in human cells
    Depositor
    Roberto Dominguez
    Insert
    Actin gamma 2, smooth muscle (ACTG2 Human)
    Use
    Tags
    HIS-FLAG-TEV
    Expression
    Bacterial and Mammalian
    Mutation
    R257C
    Promoter
    CMV
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228911-plasmid-map-sequence-id-457665

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  8. pJCX3-ACTG2_WT

    Plasmid
    #228910
    Purpose
    Expresses wildtype smooth muscle actin gamma-2 in human cells
    Depositor
    Roberto Dominguez
    Insert
    Actin gamma 2, smooth muscle (ACTG2 Human)
    Use
    Tags
    HIS-FLAG-TEV
    Expression
    Bacterial and Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228910-plasmid-map-sequence-id-457664

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  10. pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50466
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50466-plasmid-map-sequence-id-305068

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  12. pBa-MARK2-eGFP

    Plasmid
    #66706
    Purpose
    mammalian expression vector for MARK2 with a C-terminal eGFP
    Depositor
    Gary Banker, Marvin Bentley
    Insert
    MARK2 (MARK2 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    chicken beta actin
    Available since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    66706-plasmid-map-sequence-id-114008

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  13. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Expression
    Mutation
    Promoter
    TgTUB1 and Toxoplasma U6
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  14. BRAF in pENTR

    Plasmid
    #216605
    Purpose
    gateway cloning donor vector containing BRAF
    Depositor
    Douglas Allan, Christopher Loewen
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216605-plasmid-map-sequence-id-427163

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  15. p5E-2.3k-fabp2

    Plasmid
    #84927
    Purpose
    –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector
    Depositor
    John Rawls
    Insert
    –2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
    Use
    Tags
    NO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    84927-plasmid-map-sequence-id-254043

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  16. pAAV-DIO-CAG-TVA-P2A-dTomato

    Plasmid
    #177016
    Purpose
    For expression of the avian TVA receptor in a cre-dependent manner
    Depositor
    Peter Jonas
    Insert
    TVA-P2A-dTomato (CD320 Coturnix japonica)
    Use
    AAV and Cre/Lox
    Tags
    dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177016-plasmid-map-sequence-id-353739

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  17. pFastBac_Dual_SNF2L-opt_p48

    Plasmid
    #102243
    Purpose
    Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual
    Depositor
    Joe Landry
    Inserts
    SNF2L (SMARCA1 Human)
    pRbAp48 (RBBP4 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    codon optimized for expression
    Promoter
    Polyhedrin and p10
    Available since
    Nov. 1, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    102243-plasmid-map-sequence-id-197769

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  19. pmTurquoise2-Drp1

    Plasmid
    #110946
    Purpose
    Expresses dynamin-like protein 1 fused to mTurquoise2
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Dynamin-1-like protein (DNM1L Human)
    Use
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    110946-plasmid-map-sequence-id-211347

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  21. CTNNB1 in pENTR

    Plasmid
    #216617
    Purpose
    gateway cloning donor vector containing CTNNB1
    Depositor
    Douglas Allan, Christopher Loewen
    Insert
    CTNNB1 (CTNNB1 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216617-plasmid-map-sequence-id-427248

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  22. pAG416-GAL-FUS-G156E

    Plasmid
    #181716
    Purpose
    Galactose inducible expression of FUS-G156E
    Depositor
    Alejandro Chavez
    Insert
    FUS (FUS Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    G156E
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181716-plasmid-map-sequence-id-430956

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  24. JMC044-B12

    Plasmid
    #58752
    Purpose
    bacterial expression of PDB 4NW3
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4NW3 (KMT2A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. pET-21a(+)-BMTU UDG-6xHis

    Plasmid
    #172197
    Purpose
    expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions
    Depositor
    Andrea Pauli
    Insert
    BMTU UDG
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    172197-plasmid-map-sequence-id-345439

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  26. p-dCas9-TET1-Hygro

    Plasmid
    #104404
    Purpose
    transient expression of dCas9-TET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    TET1 (TET1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104404-plasmid-map-sequence-id-204454

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  27. ACACA

    Plasmid
    #39083
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACACA (ACACA Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid
    #230055
    Purpose
    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230055-plasmid-map-sequence-id-457173

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  29. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50463
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50463-plasmid-map-sequence-id-75416

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  30. Hs.KRAS4b G13D

    Plasmid
    #83133
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G13D
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83133-plasmid-map-sequence-id-158096

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  32. AICSDP-35:AAVS1-mEGFP

    Plasmid
    #91565
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    91565-plasmid-map-sequence-id-456463

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  34. DYRK1A

    Plasmid
    #38913
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DYRK1A (DYRK1A Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50470
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50470-plasmid-map-sequence-id-75489

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  36. 4IKP

    Plasmid
    #60080
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CARM1:JMC049-A06:C226836 (CARM1 Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Expression
    Mutation
    See comments
    Promoter
    polyhedrin
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. Hs.KRAS4b G12D

    Plasmid
    #83131
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12D
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83131-plasmid-map-sequence-id-158094

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  38. Hs.KRAS4b G12S

    Plasmid
    #83144
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12S mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12S
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83144-plasmid-map-sequence-id-158107

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  39. pGFP Cas

    Plasmid
    #50729
    Purpose
    mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    Cas (Bcar1 Rat)
    Use
    Tags
    FLAG and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50729-plasmid-map-sequence-id-340937

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  40. Hs.KRAS4b

    Plasmid
    #83129
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83129-plasmid-map-sequence-id-158092

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Showing: 6241 - 6280 of 8366 results