We narrowed to 4,419 results for: 424

1. TetO-FUW-Mecom 1

   Plasmid

   #118590

   Purpose

   Expresses Mecom isoform 1 from the TRE3G promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Mecom (Mecom Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

   Plasmid

   #174633

   Purpose

   Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Tags

   mCherry-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF0825

   Plasmid

   #141721

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LHX8 (LHX8 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPU-huMDA5-miR30-MDA5

   Plasmid

   #174224

   Purpose

   All-in-one huMDA5 rescue-MDA5 shRNA knockdown vector

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   MDA5 (IFIH1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGex-4T-2_GEC1

   Plasmid

   #73945

   Purpose

   For recombinant expression of human GEC1/GABARAPL1 in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   GEC1 (GABARAPL1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-GFP-IMP2-2_antisense-control

   Plasmid

   #42174

   Depositor

   Alexandra Kiemer

   Article

   Kessler et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

7. mCardinal-MCAK-C-7

   Plasmid

   #56168

   Purpose

   Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   MCAK (KIF2C Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

   Plasmid

   #32008

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   Lipin1 (Lpin1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

   Promoter

   CMV, SP6

   Available Since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pODN-mNG-ECT2

   Plasmid

   #183835

   Purpose

   Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 homology arms with mNeonGreen-linker (ECT2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF1493

    Plasmid

    #143286

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOSL1 (FOSL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-NCBD + ACTR

    Plasmid

    #99335

    Purpose

    Bacterial coexpression of CBP NCBD and NCoA3 binding domains

    Depositor

    Peter Wright

    Article

    Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

    Inserts

    CBP NCBD (2059-2117) (CREBBP Mouse)
    SRC3 (1023-1093) (NCOA3 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)

    Plasmid

    #174634

    Purpose

    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Tags

    mCherry-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ADRA2A-DuET

    Plasmid

    #213177

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADRA2A (ADRA2A Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TetO-FUW-Mecom 2

    Plasmid

    #118591

    Purpose

    Expresses Mecom isoform 2 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Mecom (Mecom Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRC55-GFP-DHX37

    Plasmid

    #192150

    Purpose

    express GFP-DHX37

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Insert

    GFP-DHX37 (Dhx37 Mouse)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0636

    Plasmid

    #142282

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXC5 (HOXC5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXS-IRES-Blast POLD1

    Plasmid

    #160805

    Purpose

    Express POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Retroviral

    Mutation

    Codon optimized C-terminal 230 amino acids

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2068

    Plasmid

    #143335

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FLII (FLII Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-CTLA4-FLN-ELP-His-ybbr

    Plasmid

    #168038

    Purpose

    Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Cytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
    

    Tags

    3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MAP3K13 gRNA (BRDN0001145716)

    Plasmid

    #76904

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP3K13

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP3K13 (MAP3K13 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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