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Plasmid#192402PurposeOn state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.DepositorInsertFRT-Act2-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ
Plasmid#192405PurposeOff state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.DepositorInsertB3RT-Act2-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ
Plasmid#192403PurposeOff state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.DepositorInsertFRT-Act2-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ
Plasmid#192381PurposeTo test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ
Plasmid#192379PurposeTo test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-13aa-Venus-pEGFP-C3
Plasmid#166753PurposeFor expression of mCerulean3 linked to Venus by 13 amino acid linkerDepositorInsertmCerulean3-13aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-39aa-Venus-pEGFP-C3
Plasmid#166755PurposeFor expression of mCerulean3 linked to Venus by 39 amino acid linkerDepositorInsertmCerulean3-39aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-31aa-Venus-pEGFP-C3
Plasmid#166754PurposeFor expression of mCerulean3 linked to Venus by 31 amino acid linkerDepositorInsertmCerulean3-31aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTC366
Plasmid#91215Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWN10042
Plasmid#89052PurposeGF-ori-FP plasmid for GFxFP assayDepositorInsertsEGFP part 1
EGFP part 2
UseCRISPRTagsExpressionMammalianMutationPromoterCAG and noneAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Banshee-ShCit-A
Plasmid#85216PurposeShort hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.DepositorInsertshCit-A
UseRetroviralTagsExpressionMammalianMutationPromoterH1Available sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Banshee-ShCit-B
Plasmid#85217PurposeShort hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.DepositorInsertshCit-B
UseRetroviralTagsExpressionMammalianMutationPromoterH1Available sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
refGFP1_pQE30
Plasmid#48644PurposeBact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFPDepositorInsertrefGFP1
UseTags6xHisExpressionBacterialMutationrefGFP C147S, C204QPromoterAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE155 XDpr-delta PDZB-CS2+
Plasmid#16767DepositorInsertXDpr-delta PDZB (dact1-b Frog)
UseXenopus expressionTagsExpressionMutationlast four amino acids deletedPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-GFP
Plasmid#58976Purposeexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorInsertEGFP
UseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO eNpHR 3.0-EYFP
Plasmid#26966PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibitionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationER export at 3' end of gene. Trafficking sig…PromoterEF1alphaAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1-WT 6KR
Plasmid#17560DepositorInsertFoxo1 6KR (Foxo1 Mouse)
UseTagsFlag and MycExpressionMammalianMutationK242, K245, K259, K262, K271 and K291 were replac…PromoterCMVAvailable sinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1ADA(pCMV5)
Plasmid#12149DepositorInsertFoxo1 ADA (Foxo1 Mouse)
UseTagsFlag and MycExpressionMammalianMutationThreonine 24 to Alanine (A) and Serine 253 to Asp…PromoterCMVAvailable sinceJune 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1-WT 6KQ
Plasmid#17562DepositorInsertFoxo1 6KQ (Foxo1 Mouse)
UseTagsFlag and MycExpressionMammalianMutationK242, K245, K259, K262, K271 and K291 were replac…PromoterCMVAvailable sinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/Ecad-tdTomato
Plasmid#101278PurposeLentiviral expression of shRNA targeting Ecad and Ecad-tdtomatoDepositorInsertE-cadherin
UseLentiviralTagstdtomatoExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-MAPKAPK2
Plasmid#82720PurposeExpresses human MAPKAPK2DepositorInsertMAPKAPK2 (MAPKAPK2 Human)
UseTags3xFLAG and GSTExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRV FOXP3 WRR
Plasmid#13624DepositorInsertFOXP3 (FOXP3 Human)
UseRetroviralTagsMycExpressionMammalianMutationWRR: N361W, E399R, E401RPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag HBx (G124L, I127A)
Plasmid#42598DepositorInsertHepatitis B virus protein X (G124L, I127A)
UseTagsFlagExpressionMammalianMutationG124L I127APromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX458-sgRNA_Ago1_1
Plasmid#73533PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1DepositorInsertAGO1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-sgRNA_Ago1_2
Plasmid#73534PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1DepositorInsertAGO1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIneoLuc-PP1A
Plasmid#128348PurposeVector for LUMIER assayDepositorInsertPP1A (PPP1CA Human)
UseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX458-sgRNA_Ago2_3
Plasmid#73531PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago2DepositorInsertAGO2
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-sgRNA_Ago2_4
Plasmid#73532PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago2DepositorInsertAGO2
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-sgRNA_Ago1_3
Plasmid#73535PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1DepositorInsertAGO1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR FOXP3 WWRR
Plasmid#16364DepositorInsertFOXP3 (FOXP3 Human)
UseTagsExpressionBacterialMutationWWRR: T359W, N361W, E399R, E401RPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only