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Showing: 6281 - 6320 of 10096 results

  5. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pCAGG-NFIX1

    Plasmid
    #112702
    Purpose
    Expression of NFIX1 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I X1 (Nfix Mouse)
    Use
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112702-plasmid-map-sequence-id-212396

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  8. pENTR11 CA NFAT1

    Plasmid
    #11792
    Depositor
    Anjana Rao
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Tags
    HA
    Expression
    Bacterial
    Mutation
    Constitutively active: multiple serine to alanine…
    Promoter
    Available since
    Aug. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    11792-plasmid-map-sequence-id-229776

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  9. pGW_RUBY_EggCas9

    Plasmid
    #225990
    Purpose
    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225990-plasmid-map-sequence-id-448019

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  10. pOpen-T4gene30

    Plasmid
    #165554
    Purpose
    Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    T4-DNA Ligase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165554-plasmid-map-sequence-id-339904

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  11. Banshee Runx1FL mCherry

    Plasmid
    #80157
    Purpose
    retroviral expression of Runx1Fl and mCherry
    Depositor
    Ellen Rothenberg
    Insert
    Runx1 (Runx1 Mouse)
    Use
    Retroviral
    Tags
    HA and IRES-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    80157-plasmid-map-sequence-id-153318

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  12. PX404 Campylobacter jejuni Cas9

    Plasmid
    #68338
    Purpose
    Campylobacter jejuni Cas9
    Depositor
    Feng Zhang
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    HA-NLS and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68338-plasmid-map-sequence-id-119561

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  13. pCAGG-NFIB2

    Plasmid
    #112700
    Purpose
    Expression of NFIB2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I B2 (Nfib Mouse)
    Use
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112700-plasmid-map-sequence-id-212390

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  14. pFRT-TODestFLAGHAhTTP

    Plasmid
    #48738
    Purpose
    Expresses hTTP
    Depositor
    Thomas Tuschl
    Insert
    TTP (ZFP36 Human)
    Use
    Tags
    FLAG, HA, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    48738-plasmid-map-sequence-id-70782

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  15. pLenti-ePEG-LINE1-loxPsym

    Plasmid
    #216866
    Purpose
    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements
    Depositor
    Leopold Parts
    Insert
    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216866-plasmid-map-sequence-id-441211

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  16. pMT ERK1

    Plasmid
    #12656
    Depositor
    John Kyriakis
    Insert
    ERK1 (Mapk3 Rat)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 21, 2006
    Availability
    Academic Institutions and Nonprofits only

  17. pKT-PDGFRa-IRES-Katushka

    Plasmid
    #133081
    Purpose
    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    PDGFRa (Pdgfra Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    133081-plasmid-map-sequence-id-261534

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  18. YARS_pNIC-CTB10H

    Plasmid
    #153059
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    YARS (YARS1 Human)
    Use
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153059-plasmid-map-sequence-id-304479

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  19. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  20. pGHE-PdNIP2-1

    Plasmid
    #108447
    Purpose
    PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.
    Depositor
    Richard Bélanger
    Insert
    PdNIP2-1
    Use
    Tags
    Expression
    Mutation
    Promoter
    pT7
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    108447-plasmid-map-sequence-id-274297

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  21. PX381 Filifactor alocis Cas9

    Plasmid
    #68318
    Purpose
    Filifactor alocis Cas9
    Depositor
    Feng Zhang
    Insert
    FaCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68318-plasmid-map-sequence-id-119531

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  22. MT4_C30Y R31W

    Plasmid
    #105712
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 3 subisoform with C30Y and R31W substitution
    Depositor
    Artur Krężel
    Insert
    Metallothionein 4 (MT4 Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    This subisoform has a C30Y and R31W substitution
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105712-plasmid-map-sequence-id-203330

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  23. PX385 Streptococcus pasteurianus Cas9

    Plasmid
    #68322
    Purpose
    Streptococcus pasteurianus Cas9
    Depositor
    Feng Zhang
    Insert
    SpaCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68322-plasmid-map-sequence-id-119537

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  24. PX396 Parvibaculum lavamentivorans Cas9

    Plasmid
    #68333
    Purpose
    Parvibaculum lavamentivorans Cas9
    Depositor
    Feng Zhang
    Insert
    PlCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68333-plasmid-map-sequence-id-119549

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  25. PX377 Corynebacter diphtheriae Cas9

    Plasmid
    #68314
    Purpose
    Corynebacter diphtheriae Cas9
    Depositor
    Feng Zhang
    Insert
    CdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68314-plasmid-map-sequence-id-119522

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  26. PX405 Neisseria meningitidis Cas9

    Plasmid
    #68702
    Purpose
    Neisseria meningitidis Cas9
    Depositor
    Feng Zhang
    Insert
    NmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. PX394 Neisseria cinerea Cas9

    Plasmid
    #68331
    Purpose
    Neisseria cinerea Cas9
    Depositor
    Feng Zhang
    Insert
    NcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68331-plasmid-map-sequence-id-119554

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  28. PX406 Pasteurella multocida Cas9

    Plasmid
    #68703
    Purpose
    Pasteurella multocida Cas9
    Depositor
    Feng Zhang
    Insert
    PmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. PX390 Flavobacterium columnare Cas9

    Plasmid
    #68327
    Purpose
    Flavobacterium columnare Cas9
    Depositor
    Feng Zhang
    Insert
    FcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68327-plasmid-map-sequence-id-119543

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  30. PX383 Lactobacillus johnsonii Cas9

    Plasmid
    #68320
    Purpose
    Lactobacillus johnsonii Cas9
    Depositor
    Feng Zhang
    Insert
    LjCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68320-plasmid-map-sequence-id-119535

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  32. pCMV-NFIB2ERt2

    Plasmid
    #112703
    Purpose
    Expression of NFIB2-ERT2 fusion protein
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I B2 (Nfib Mouse)
    Use
    Tags
    ERT2 and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112703-plasmid-map-sequence-id-214845

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  33. PX386 Lactobacillus farciminis Cas9

    Plasmid
    #68323
    Purpose
    Lactobacillus farciminis Cas9
    Depositor
    Feng Zhang
    Insert
    LfCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68323-plasmid-map-sequence-id-119538

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  34. PX395 Roseburia intestinalis Cas9

    Plasmid
    #68332
    Purpose
    Roseburia intestinalis Cas9
    Depositor
    Feng Zhang
    Insert
    RiCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68332-plasmid-map-sequence-id-119548

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  36. AmCyan-P2A-Xerry

    Plasmid
    #48686
    Purpose
    Expresses AmCyan while the mCherry gene has been mutated
    Depositor
    Ilpo Huhtaniemi
    Insert
    AmCyan
    Use
    Tags
    mutant mCherry (truncated)
    Expression
    Mammalian
    Mutation
    SV40 NLS
    Promoter
    CMV
    Available since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    48686-plasmid-map-sequence-id-70482

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  37. LHP599 - pACT2-Ub

    Plasmid
    #32170
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    Gal4 AD and HA
    Expression
    Yeast
    Mutation
    Promoter
    pADH1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  38. PX389 Fluviicola taffensis Cas9

    Plasmid
    #68326
    Purpose
    Fluviicola taffensis Cas9
    Depositor
    Feng Zhang
    Insert
    FtCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68326-plasmid-map-sequence-id-119542

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  39. PX388 Bacteroides coprophilus Cas9

    Plasmid
    #68325
    Purpose
    Bacteroides coprophilus Cas9
    Depositor
    Feng Zhang
    Insert
    BcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68325-plasmid-map-sequence-id-125196

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  40. PX378 Sutterella wadsworthensis Cas9

    Plasmid
    #68315
    Purpose
    Sutterella wadsworthensis Cas9
    Depositor
    Feng Zhang
    Insert
    SwCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68315-plasmid-map-sequence-id-119526

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Showing: 6281 - 6320 of 10096 results