We narrowed to 10,458 results for: T7

1. pDEST17-ScChd1[Y137A]

   Plasmid

   #221198

   Purpose

   ScChd1-aa118-1274 chromatin remodeler with Y137A mutation

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   ScChd1 (aa118-1274)
   

   Use

   Tags

   6xHis-tag (Precission protease cleavable)

   Expression

   Bacterial

   Mutation

   Y137A

   Promoter

   T7

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-H2-18 Heavy chain-SpyTag003

   Plasmid

   #216307

   Purpose

   Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-Tras heavy chain-SpyTag003

   Plasmid

   #216293

   Purpose

   Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDF-Leup

   Plasmid

   #213154

   Purpose

   Expresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.

   Depositor

   Jason Crawford

   Article

   Li et al J Nat Prod. 2021 Oct 22;84(10):2692-2699

   Insert

   Leupeptin Protease Inhibitor Pathway
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28_Caspase-11_humanized-mutant

   Plasmid

   #214315

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (Casp4 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   N152K_KLS288YKT_KASIHS348TPRAKA

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28_Caspase-4_W267N

   Plasmid

   #214314

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   W267N

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28_Caspase-4_R269D

   Plasmid

   #214313

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   R269D

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR-D-Topo-kcnk17

   Plasmid

   #208999

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk17 (kcnk17 Zebrafish)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR221-kcnk2a

   Plasmid

   #208983

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk2a (kcnk2a Zebrafish)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221-kcnk6

    Plasmid

    #208990

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk6 (kcnk6 Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221-kcnk13a

    Plasmid

    #208996

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk13a (kcnk13a Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST-SHmOCR

    Plasmid

    #206874

    Purpose

    Empty Gateway pDEST vector for bacterial expression with Super His - Monomeric OCR fusion

    Depositor

    Murray Junop

    Article

    Szabla et al Nucleic Acids Res. 2024 Jul 22:gkae635.

    Type

    Empty backbone

    Use

    Tags

    HSV + 6xHis and SuperHis (14xHis) + monomeric OCR…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30b jGCaMP8f L27A

    Plasmid

    #204138

    Purpose

    Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Use

    Tags

    N-terminal His tag

    Expression

    Bacterial

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    T7

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. nUnaG-ST

    Plasmid

    #207630

    Purpose

    A plasmid encoding the N-terminal UnaG fragment fused to SpyTag.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    nUnaG-SpyTag-MBP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 1-84

    Promoter

    T7

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SpyCatcher-DIIG

    Plasmid

    #201196

    Purpose

    Expresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteria

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

    Insert

    SpyCatcher-DIIG
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SpyCatcher-SWG

    Plasmid

    #201194

    Purpose

    Expresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteria

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

    Insert

    SpyCatcher-SWG
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpyCatcher-PEED

    Plasmid

    #201195

    Purpose

    Expresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteria

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

    Insert

    SpyCatcher-PEED
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1BR9-BFPSTOP

    Plasmid

    #202055

    Purpose

    E. coli expression vector for N-ter GFP11 R9 tagged BFP

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    BFP
    

    Use

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST17-HO1(51-231)

    Plasmid

    #203486

    Purpose

    For recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    Arabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRRL-VinculinI997A_TurboID_Venus

    Plasmid

    #200273

    Purpose

    Lentivirus vector expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

    Depositor

    Brenton Hoffman

    Article

    Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

    Insert

    VinculinI997A_TurboID_Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28b_TypeIC_acrIF2_invivo

    Plasmid

    #196402

    Purpose

    expresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay

    Depositor

    David Taylor

    Article

    O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

    Insert

    anti-CRISPR IF2 protein (orf30 P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28b_TypeIC_acrIC4_invivo

    Plasmid

    #196403

    Purpose

    expresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assay

    Depositor

    David Taylor

    Article

    O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

    Insert

    anti-CRISPR IC4 protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. eSrtA-MT4

    Plasmid

    #200306

    Purpose

    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    eSrtA-LPETG-MT4 (MT4 Human)
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. eSrtA-MT3

    Plasmid

    #200305

    Purpose

    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    eSrtA-LPETG-MT3 (MT3 Human)
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCA528 AMSHLP 16-218

    Plasmid

    #180609

    Purpose

    Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    AMSHLP residues 16-218
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 16-218

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJL1_SUL-I

    Plasmid

    #200172

    Purpose

    Amino acids 25-308 of SUL-I with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

    Depositor

    Michael Jewett

    Article

    Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

    Insert

    SUL-I
    

    Use

    Tags

    6x His tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NeuA.pJL1

    Plasmid

    #199115

    Purpose

    In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)

    Depositor

    Michael Jewett

    Article

    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

    Insert

    N-acylneuraminate cytodiylyltransfease (NeuA)
    

    Use

    Tags

    Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Derp2.pJL1

    Plasmid

    #199116

    Purpose

    In vitro expression of Der p 2, a major common allergen from Dermatophagoides pteronyssinus (dust mite), with synthetic site for glycosylation encoded (GGNWTT)

    Depositor

    Michael Jewett

    Article

    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

    Insert

    Der p 2 dust mite allergen
    

    Use

    Tags

    Glyctag, His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pQE80l BCB

    Plasmid

    #196472

    Purpose

    Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

    Depositor

    Fei Sun

    Article

    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

    Insert

    BCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p32m3C Trx-CN

    Plasmid

    #196474

    Purpose

    Protein expression of Thiolredoxin-3C cleavage site-CarHCN

    Depositor

    Fei Sun

    Article

    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

    Insert

    Trx-CN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pQE80l AEC

    Plasmid

    #196473

    Purpose

    Protein expression of SpyTag-ELP-CarHC

    Depositor

    Fei Sun

    Article

    Yang et al Matter (2020) 2, 233–249

    Insert

    AEC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSFDuet-1_Uricase

    Plasmid

    #182400

    Purpose

    Bacterial expression of uricase

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    uricase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSLS.539

    Plasmid

    #184958

    Purpose

    Test effect of a1/a2 length on editing fabH using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    fabH donor G954A

    Promoter

    T7/lac

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRD411

    Plasmid

    #178196

    Purpose

    sRok overexpression

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    srok
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRD231

    Plasmid

    #178195

    Purpose

    Rok overexpression

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    rok
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENT-L3-eBra-L4

    Plasmid

    #186368

    Purpose

    Entry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Brachury notochord enhancer
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pENT-L3-Titf-L4

    Plasmid

    #186367

    Purpose

    Entry clone with ORF encoding Titf1 cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Titf endoderm enhancer
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPgiCas13b

    Plasmid

    #184833

    Purpose

    Inducible expression of PgiCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Cas13b nuclease from Porphyromonas gingivalis
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSLS.543

    Plasmid

    #184960

    Purpose

    Test effect of a1/a2 length on editing murF using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    murF donor G1359A

    Promoter

    T7/lac

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSLS.545

    Plasmid

    #184962

    Purpose

    Test effect of a1/a2 length on editing priB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    priB donor G1069A

    Promoter

    T7/lac

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge