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pET20b-delta133HtrA2 S400D

Plasmid

#16156

Depositor

L. Miguel Martins

Publication

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

S400D

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pJS2

Plasmid

#253727

Purpose

CRISPRi system for Pseudomonas aeruginosa expressing sgRNA from the rhamnose-inducible rhaBAD promoter

Depositor

Deborah Hung

Publication

Sullivan et al mBio. 2026 Feb 11:e0276725. doi: 10.1128

Type

Empty backbone

Expression

Bacterial

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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Venus-MOA

Plasmid

#234741

Purpose

Mammalian expression of mitochondria marker Venus-MOA

Depositor

Nevin Lambert

Publication

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-MOA

Expression

Mammalian

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_SeV_RdRP

Plasmid

#228808

Purpose

Expression of Sendai virus RdRP

Depositor

Shravanti Rampalli

Publication

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

RdRP

Tags

EGFP

Expression

Mammalian

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJMP12049

Plasmid

#222355

Purpose

Tn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.

Depositor

Jason Peters

Publication

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1-Nir3-LNS2

Plasmid

#220087

Purpose

C-terminal PA binding domain of Nir3

Depositor

Gerry Hammond

Publication

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM2 (PITPNM2 Human)

Tags

mEGFP-GGGGSHM

Expression

Mammalian

Mutation

amino acids 925-1209

Promoter

CMV

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-rTOM20-TagBFP

Plasmid

#210332

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Publication

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Tags

TagBFP

Expression

Mammalian

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-rTOM20-GFP

Plasmid

#210331

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Publication

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Tags

GFP

Expression

Mammalian

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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plvx-mTSPAN9-mCherry

Plasmid

#210337

Purpose

Overexpression of mTSPAN9

Depositor

Li Yu

Publication

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mTSPAN9 (Tspan9 Mouse)

Tags

mCherry

Expression

Mammalian

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_AC

Plasmid

#148417

Purpose

Mammalian Expression of HsRck

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck (DDX6 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAB167

Plasmid

#101672

Purpose

Opto-T7RNAP*(563), equal expr.

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB152

Plasmid

#101663

Purpose

Opto-T7RNAP*(563-F2)

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2)

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAB147

Plasmid

#101662

Purpose

Opto-T7RNAP*(302)

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(302)

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB164

Plasmid

#101667

Purpose

Opto-T7RNAP*(563), half expr.

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGST2-TSG101 (2-145)

Plasmid

#184810

Purpose

Bacterial expression of TSG101 UEV domain (aa 2-145)

Depositor

James Hurley

Publication

Im et al Structure. 2010 Nov 10. 18(11):1536-47.

Insert

TSG101 (TSG101 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-145 only

Promoter

tac

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

Plasmid

#183077

Purpose

Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

Depositor

Jennifer Doudna

Publication

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and crRNA ending with a HDV ribozyme

Use

CRISPR

Expression

Bacterial

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVP16 AD

Plasmid

#165822

Purpose

VP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

VP16 AD

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniWhiteA2

Plasmid

#165873

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-C93A

Plasmid

#133910

Purpose

Bacterial expression of His6-Ubc9-C93A

Depositor

Jeffrey Bode

Publication

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 93 to Alanine

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pNLRP11major.MYC

Plasmid

#131196

Purpose

Mammalian expression of human NLRP11 188S

Depositor

Thomas Kufer

Publication

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188S (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188S

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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