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pcDNA3-Myc:P4:gs:nls:ABI

Plasmid

#135985

Purpose

Expresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the ABI domain

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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OVA-miSFIT-17-T

Plasmid

#124677

Purpose

Tuning expression of cytosolic Ovalbumin coupled to EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

Cyto-Ovalbumin

Expression

Mammalian

Mutation

Truncated OVA for cytoplasmic expression

Promoter

minimal CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1.miR16

Plasmid

#128747

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA TdR

Plasmid

#80940

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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eNme2-C TadDE

Plasmid

#193842

Purpose

Express TadDE (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

eNme2-C TadDE

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_SPIB_P2A_Hygro_Barcode

Plasmid

#120483

Purpose

Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIB (SPIB Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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WT (AF20)

Plasmid

#21359

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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PGK+ episome

Plasmid

#170041

Purpose

PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK-tetO2

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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WT (REP10)

Plasmid

#21368

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Expression

Mammalian

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Rab5-Blasticidin

Plasmid

#120715

Purpose

Expresses early endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab5a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Amino acids 2-215

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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CMV+ episome

Plasmid

#169742

Purpose

CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Ef1a_LIN28_Ires_Puro

Plasmid

#18921

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

LIN28 (LIN28A Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pMRX-IZ-ANXA4-mRuby3

Plasmid

#184890

Purpose

Stable expression of ANXA4-mRuby3 (Annexin A4-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA4 (ANXA4 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-CMP-PE-V1

Plasmid

#202769

Purpose

Expresses CMP-PE-V1 in mammalian cells for prime editing

Depositor

Kyoungmi Kim

Article

Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

Insert

CMP-PE-V1

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Rab11-Blasticidin

Plasmid

#120717

Purpose

Expresses recycling endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab11a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Amino acids 2-216

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-CaMKIID-K43M

Plasmid

#186830

Purpose

Expresses FLAG-CaMKIID-K43M in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2d (Camk2d Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 43 to Methionine

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Chronos-mT-Sapphire-NRN

Plasmid

#149692

Purpose

For packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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Tmem214 pmRFP-N2 (1348)

Plasmid

#62047

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pEGIP*35

Plasmid

#26776

Purpose

Enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

GFP*35

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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AsCas12a-DR-cr in PBCSK-

Plasmid

#126640

Purpose

Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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