We narrowed to 39,967 results for: lat

1. TIMP1-bio-His

   Plasmid

   #52089

   Purpose

   Expresses full-length Metalloproteinase inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TIMP1 (TIMP1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6p-1-CDK5RAP3

   Plasmid

   #204566

   Purpose

   Expresses GST-CDK5RAP3 in E.coli

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CDK5RAP3 (AMY2A Human)
   

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG MCP-VP64

   Plasmid

   #92357

   Purpose

   CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   MCP-VP64
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

   Plasmid

   #121976

   Purpose

   transfection studies in eukaryotic cells such as COS-7 cells

   Depositor

   Christophe Erneux

   Article

   De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

   Insert

   Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)
   

   Expression

   Mammalian

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3_altPKA-Cα-mEGFP

   Plasmid

   #114132

   Purpose

   Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   18747 (Prkaca Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiMutB3GALT5catdead-blast

   Plasmid

   #220869

   Purpose

   lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   encodes D156A and D243A mutations; also, nucleoti…

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5 MEKK2 WT

   Plasmid

   #12182

   Depositor

   Gary Johnson

   Article

   Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

   Insert

   MEKK2 (Map3k2 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

8. TIMP3-bio-His

   Plasmid

   #53418

   Purpose

   Expresses full-length Metalloproteinase inhibitor 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TIMP3 (TIMP3 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-LacR-TopBP1

   Plasmid

   #31313

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

   Insert

   TopBP1 (TOPBP1 Human)
   

   Tags

   FLAG and LacR

   Expression

   Mammalian

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pPAICS-2XStrep

    Plasmid

    #125683

    Purpose

    expresses human PAICS protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    PAICS (PAICS Human)
    

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSaPIPLC His

    Plasmid

    #173810

    Purpose

    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

    Insert

    Staphylococcus aureus plc
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSV-CaMKIV

    Plasmid

    #45062

    Purpose

    Expression of CaMKIV

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Cam Kinase IV (Camk4 Mouse)
    

    Expression

    Mammalian

    Mutation

    *

    Promoter

    RSV

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. SYMPK

    Plasmid

    #156071

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SYMPK (SYMPK Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-cTNT-Cre-miR122 TS

    Plasmid

    #209784

    Purpose

    Expresses Cre by the specific cTNT promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    miR122 ts
    

    Use

    AAV

    Promoter

    cTNT

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Str-STIM1-NN_ST-SBP-mCherry

    Plasmid

    #65263

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-C1-hArfaptin1

    Plasmid

    #206404

    Purpose

    Mammalian expression of hArfaptin1

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

    Insert

    Arfaptin1 (ARFIP1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRB117.2

    Plasmid

    #181949

    Purpose

    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 narL - E. coli, Synthetic)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTET2-RlucCP

    Plasmid

    #198352

    Purpose

    Part of Firefly reporter system, encodes Renilla luciferase

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    pTET2

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PRPF18

    Plasmid

    #155846

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PRPF18 (PRPF18 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLL5.0 hCortKD EGFP

    Plasmid

    #187267

    Purpose

    Lentiviral expression of shRNA targeting Cortactin

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    shRNA for Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    U6

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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