We narrowed to 7,200 results for: mme

1. pCDH-EF1a-tetR-T2A-tSapphire

   Plasmid

   #176312

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   T-sapphire
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB1A3 EcoRI Methylase-AmilCP

   Plasmid

   #85166

   Purpose

   For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

   Depositor

   Robin Dowell

   Article

   Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

   Insert

   EcoRI Methylase-AmilCP
   

   Tags

   AmilCP

   Expression

   Bacterial

   Promoter

   J23100 promoter

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

3. SIN40C.SFFV.dTomato.miR-ctrl

   Plasmid

   #169303

   Purpose

   Constitutive overexpression of miR-ctrl with dTomato as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   miR-ctrl
   

   Use

   Lentiviral

   Tags

   dTomato

   Promoter

   SFFV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. KTK_001

   Plasmid

   #180517

   Purpose

   Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TR- ATG-HP-GFP

   Plasmid

   #80592

   Purpose

   Expresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   GFP
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Inserted 30 nt hairpin which is targeted by Csy4 …

   Promoter

   CBA

   Available Since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNIR-Fb6m

   Plasmid

   #184680

   Purpose

   NIR-Fb to spike-Cov-19 RBD

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fb6m (S Synthetic)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CD23b in pIRES2eGFP

   Plasmid

   #19136

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   CD23 beta (FCER2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pLL7.0: Venus-oLID-CAAX (from KRas4B)

   Plasmid

   #60412

   Purpose

   Mammalian Expression of Venus-oLID-CAAX

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   Venus-oLID-CAAX
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. gRD21A-mRFP

   Plasmid

   #181959

   Purpose

   RD21A protein tagged with mRFP, under control of native promoter

   Depositor

   George Haughn

   Article

   Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

   Insert

   RD21A genomic sequence including 2kb upstream of the ATG
   

   Tags

   mRFP

   Expression

   Plant

   Promoter

   RD21A native

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti_CRISPRi_sgNeg2

    Plasmid

    #166999

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 2
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTK157_mCherry-4.1G-CTD

    Plasmid

    #46359

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G C-terminal domain (EPB41L2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C-terminal domain from 886-1005 amino acids

    Promoter

    CMV

    Available Since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEYFP-N1-hRIP3-FL V460P

    Plasmid

    #61379

    Purpose

    Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with V460Pmutation (RIPK3 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V460 to P

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pEYFP-N1-hRIP3-FL V458P

    Plasmid

    #61377

    Purpose

    Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with V458Pmutation (RIPK3 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V458 to P

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-FLAG-Fos DD T325A T331A

    Plasmid

    #8973

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362D, S374D, T325A, T331A

    Available Since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pMT3-RhoGC

    Plasmid

    #100031

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoGC
    

    Tags

    1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)

    Expression

    Mammalian

    Promoter

    Adenovirus major late promoter

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYZ_NAND2_L17_S19_S11

    Plasmid

    #132730

    Purpose

    T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_NAND2_L17_S19_S11
    

    Expression

    Bacterial

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b-ecDHFR-G51PEKN

    Plasmid

    #118582

    Purpose

    Bacterial expression of E.coli DHFR G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    G51 mutated to P51; Glu-Lys-Asn residues inserted…

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-His6-OAZ-GSY-SPM-Ctag

    Plasmid

    #163613

    Purpose

    Expresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    OAZ-GSY-SPM
    

    Tags

    C-tag and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b-ecDHFR-Y100F

    Plasmid

    #110826

    Purpose

    Bacterial expression of E.coli DHFR Y100F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Mutation

    Y100 mutated to F100

    Promoter

    T7

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. A102 ΔAmpH Δ2x+ve

    Plasmid

    #129484

    Purpose

    Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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