We narrowed to 18,356 results for: Tec

1. pSBY2_ligD(FnCpf1)

   Plasmid

   #104623

   Purpose

   CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD

   Depositor

   Sheng Yang

   Article

   Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

   Insert

   crRNA to ligD
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pj23119

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. YEp112-CUP1-His-Smt3

   Plasmid

   #99537

   Purpose

   Yeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 marker

   Depositor

   Helle Ulrich

   Article

   Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LBJJ382

   Plasmid

   #117518

   Purpose

   Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCS Citrine

   Plasmid

   #91761

   Purpose

   Bacterial expression of a fluorescent protein, Citrine

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Insert

   Citrine
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay FLIPE-600n

   Plasmid

   #13545

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E WT
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1P shNFS1_1

   Plasmid

   #102963

   Purpose

   Suppression of NFS1

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shNFS1_1 (NFS1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJ915v2 + sfGFP

   Plasmid

   #88918

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1xNLS-pMJ915v2

   Plasmid

   #88915

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19/mChe

   Plasmid

   #183161

   Purpose

   Construct for transient expression of cell nucleus-, cytoplasm-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTH1-SpSrtA

    Plasmid

    #64987

    Purpose

    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    S. pyogenes sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-81

    Promoter

    T7 promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONRPEX18Km-SceI-pheS

    Plasmid

    #111610

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. DL034:cFos::HaloTag-iPAK4

    Plasmid

    #177884

    Purpose

    HaloTag-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    cFos

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB4778

    Plasmid

    #106125

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLH-sgRNA1-PP7-boxB

    Plasmid

    #75393

    Purpose

    sgRNA1-PP7-boxB

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XPP7-boxB
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDYU1B/mRuby3–α-tubulin

    Plasmid

    #154302

    Purpose

    Expresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideum

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Insert

    alpha-tubuin
    

    Use

    Dictyostelium expression

    Tags

    mRuby3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mUAVbla:tetR 5′

    Plasmid

    #149379

    Purpose

    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Insert

    tetA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTK105

    Plasmid

    #59562

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PEST

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) 2xK-turn EYFP-PEST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CP2-TCC

    Plasmid

    #179679

    Purpose

    Complementary plasmid

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [sd2] T7 RNAP-TCC linker-degron
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLH-sgRNA1-MS2-PP7

    Plasmid

    #75392

    Purpose

    sgRNA1-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XMS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YEp195-CUP1-His-Smt3

    Plasmid

    #99538

    Purpose

    Yeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 marker

    Depositor

    Helle Ulrich

    Article

    Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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