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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-NES

Plasmid

#36206

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

NES

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-SHMT2-3xFLAG

Plasmid

#233891

Purpose

lentiviral overexpression vector for SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

SHMT2 (SHMT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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hGFAP-NLS-HA-Dre-P2A-BFP

Plasmid

#51277

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Dre-P2A-BFP

Expression

Mammalian

Promoter

hGFAP

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDG04163

Plasmid

#216373

Purpose

xhNup214-Nb1t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup214-Nb1t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-scSUMO

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLPCX-NICD

Plasmid

#44471

Depositor

Ernesto Canalis

Article

Zanotti et al J Biol Chem. 2011 Feb 11;286(6):4576-88.

Insert

Notch intracellular domain (Notch1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Contains amino acid 1753 to C-Term

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-flag-mZscan4d-polyA

Plasmid

#61831

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Zscan4d (Zscan4d Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV and T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pFYF1327 EGFP Site#2

Plasmid

#47512

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pEYFP-C3-HA-Ubiquitin

Plasmid

#28236

Depositor

Michael Mancini

Article

Stenoien et al Nat Cell Biol. 2002 Oct . 4(10):806-10.

Insert

Ubiquitin (UBB Human)

Tags

EYFP and HA

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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Nanog5P reporter

Plasmid

#16337

Depositor

Austin Cooney

Article

Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

Insert

Nanog promoter (Nanog Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

Mouse Nanog promoter

Available Since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-mito

Plasmid

#113916

Purpose

Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-DeltaFosB

Plasmid

#68545

Purpose

AAV expression of DeltaFosB

Depositor

Eric Nestler

Article

Zachariou et al Nat Neurosci. 2006 Feb;9(2):205-11. Epub

Insert

DeltaFosB-IRES-GFP

Use

AAV

Expression

Mammalian

Mutation

truncated splice variant of FosB

Promoter

CMV

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only
pMCRi

Plasmid

#207524

Purpose

Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Use

Pseudomonas vector

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET29b Tau Cys

Plasmid

#108867

Purpose

Expresses human Tau with a mutated cysteine

Depositor

Gabriele Kaminski Schierle

Insert

Tau (MAPT Human)

Expression

Bacterial

Mutation

I260C C291A C322A to allow maleimide dye labelling

Promoter

T7

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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KU70-eGFP

Plasmid

#213517

Purpose

Expresses human KU70-eGFP in mammalian cells

Depositor

Karim Mekhail

Article

Shokrollahi et al bioRxiv 2023.05.07.539750

Insert

human KU70-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLD1 translation factors

Plasmid

#117760

Purpose

BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 1-13

Use

Escherichia coli

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBT346_pLambda-attB-pCA-GFP-pA

Plasmid

#52551

Purpose

for producing PhiC31 attB-containing minicircle in vitro

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

Lambda integrase attL1
Lambda integrase attR1
PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-V5-DGCR8

Plasmid

#51383

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

Plasmid

#71805

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pLD3 translation factors

Plasmid

#117762

Purpose

BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 22-30

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLD2 translation factors

Plasmid

#117761

Purpose

BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 14-21

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-CGFP-mAID

Plasmid

#140608

Purpose

Expression of OsTIR1 and mAID-GFP protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3/CyclinB1-GFP (28)

Plasmid

#39871

Depositor

Jonathon Pines

Article

Jackman et al Mol Biol Cell. 2002 Mar;13(3):1030-45.

Insert

CyclinB1 (CCNB1 Human)

Tags

GFP

Expression

Mammalian

Mutation

V5A

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCC3)

Plasmid

#235078

Purpose

Expresses Tad-CBE(GCC3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCC3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCG4)

Plasmid

#235080

Purpose

Expresses Tad-CBE(GCG4) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCG4)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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JC201: pMVP/Ad/5-35-DEST

Plasmid

#121847

Purpose

pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-cyto

Plasmid

#113914

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-H1.1

Plasmid

#32894

Depositor

Michael Hendzel

Article

Th'ng et al J Biol Chem. 2005 Jul 29;280(30):27809-1

Insert

Histone H1.1 (H1-1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2018

Availability

Academic Institutions and Nonprofits only

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pH-AFID-3

Plasmid

#167361

Purpose

For inducing predictable deletions in rice plants

Depositor

Caixia Gao

Article

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3A-Cas9-UDG-T2A-AP lyase

Expression

Plant

Mutation

WT

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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Sa-SlugCas9

Plasmid

#163797

Purpose

Expresses Sa-SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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