We narrowed to 44,081 results for: cha

1. CLPIT Cry2PHR-mCh-LRP6c

   Plasmid

   #42961

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-LRP6c
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   LPR6 cytoplasmic domain only

   Promoter

   Tet-Off promoter

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMC-1-SlUbi-6

   Plasmid

   #197709

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   SlUbi promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMC-9-SlUbi-11

   Plasmid

   #197773

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   SlUbi terminator
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-mScarlet-TEV-Strep-His

   Plasmid

   #210823

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mScarlet
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCV-PIG-miR-150

   Plasmid

   #64235

   Purpose

   Expresses miR-150 in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

   Insert

   miR150 (MIR150 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ER-GCaMP6f-AAV

   Plasmid

   #182501

   Purpose

   GFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotor

   Depositor

   Chris Richards

   Article

   Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

   Insert

   ER localized P450
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDU1

   Plasmid

   #169817

   Purpose

   pSpCas9(BB)-2A-tCD19 (pDU1)

   Depositor

   Andrea Rossi

   Article

   Ramachandran et al Life Sci Alliance. 2021 Apr 26;4(6). pii

   Insert

   tCD19
   

   Use

   Tags

   fusion protein

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF SYT1-HaloTag

   Plasmid

   #197281

   Purpose

   Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYT1 and HaloTag (Syt1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EF1alpha

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMC-1-ZmAXIG1-6

   Plasmid

   #197711

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   ZmAXIG1 promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK5-FLAG-Sestrin2-E451A

    Plasmid

    #73673

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E451A changed Glutamate 451 to Alanine

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMC-9-StUbi-11

    Plasmid

    #197774

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    StUbi terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

    Plasmid

    #13005

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 delta CC (SULF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 87 to Alanine

    Promoter

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pFLAG ARMS

    Plasmid

    #24086

    Depositor

    Moses Chao

    Article

    Kong et al J Neurosci. 2001 Jan 1. 21(1):176-85.

    Insert

    ARMS (Kidins220 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAct::Raf

    Plasmid

    #11341

    Depositor

    Michael Wigler

    Article

    Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

    Insert

    byr-2 (byr2 Fission Yeast)
    

    Use

    Tags

    Gal4 activation domain

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pGBW-m4046203

    Plasmid

    #145488

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCCL-ReDNAP (Y427A)

    Plasmid

    #52346

    Purpose

    CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

    Depositor

    Chang Liu

    Article

    Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

    Insert

    Recoded TP-DNAP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    REV1

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. A0A075HNX4

    Plasmid

    #177695

    Purpose

    expresses a fatty alcohol oxidase in E. coli

    Depositor

    Martin Engqvist

    Article

    Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

    Insert

    Long chain alcohol oxidase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSETT4gap

    Plasmid

    #153413

    Purpose

    φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

    Depositor

    Joern Kalinowski

    Article

    Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    gapDH promoter from Eggerthella lenta

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV hSynapsin flexed SV-tag

    Plasmid

    #163686

    Purpose

    express SV-Tag construct in a Cre-dependent manner

    Depositor

    Bernardo Sabatini

    Article

    Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

    Insert

    synaptophysin 9X HA
    

    Use

    AAV

    Tags

    9X HA

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRPML2-HA

    Plasmid

    #18828

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML2 (Mcoln2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

21. mPlum-H2B-6

    Plasmid

    #55976

    Purpose

    Localization: Nucleus/Histones, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Article

    Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pBac(3xP3-gTc’v; Tc-hsp68:Cre)

    Plasmid

    #119063

    Purpose

    Expression of Cre recombinase in Tribolium castaneum

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2019 Jan 4;146(1). pii: dev

    Inserts

    pBacL
    Tc-hsp68 Promoter
    Cre recombinase
    Tc-hsp68 3'UTR
    SV40polyA
    Tc-vermilion
    3xP3 TATA
    pBacR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMSCV-PIG-miR-22

    Plasmid

    #64229

    Purpose

    Expresses miR-22 in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

    Insert

    miR22 (MIR22, MIR22HG Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfB8379

    Plasmid

    #126913

    Purpose

    Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and XdCrtYB
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Kohinoor 2.0/pcDNA3

    Plasmid

    #182315

    Purpose

    Positively reversibly photoswitchable fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Wazawa et al Microscopy (Oxf). 2021 Aug 9;70(4):340-3

    Insert

    Kohinoor 2.0
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBS-KSTbx2.8.2

    Plasmid

    #42626

    Depositor

    Virginia Papaioannou

    Article

    Chapman et al Dev Dyn. 1996 Aug;206(4):379-90.

    Insert

    Tbx2 (Tbx2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

27. Dhh1-EGFP

    Plasmid

    #201418

    Purpose

    Bacterial protein expression of Dhh1

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GST-TEVcs-FIP200-MBP

    Plasmid

    #171410

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    FIP200 (RB1CC1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTR-Cdh1

    Plasmid

    #49776

    Purpose

    pENTR vector for mouse E-cadherin

    Depositor

    Jamie Davies

    Article

    Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

    Insert

    Cdh1 (Cdh1 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pECNUS_MP01

    Plasmid

    #194350

    Purpose

    CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    pcoCas9, FAST-Red
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSY14-ptb

    Plasmid

    #90490

    Purpose

    pSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

    Depositor

    Sheng Yang

    Article

    Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

    Insert

    sceC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pM423

    Plasmid

    #172408

    Purpose

    Expresses sfGFP (1-10) and MCP-GFP11

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    MCP, bipartite sfGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP {1-10}

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCR1054

    Plasmid

    #71822

    Purpose

    Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV hSynapsin SV-tag

    Plasmid

    #163685

    Purpose

    express SV-Tag construct in a Cre-independent manner

    Depositor

    Bernardo Sabatini

    Article

    Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

    Insert

    synatophysin 9X HA tag T2A tdtomato
    

    Use

    AAV

    Tags

    9X HA

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCS2 xRor2-mCherry

    Plasmid

    #67162

    Purpose

    mammalian expression / in vitro transcription

    Depositor

    Alexandra Schambony

    Article

    Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

    Insert

    Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pMDC32-SEIPIN1

    Plasmid

    #96973

    Purpose

    Express Arabidopsis SEIPIN1 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN1 (AT5G16460 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

    Plasmid

    #75295

    Purpose

    An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.

    Depositor

    Brandon Harvey

    Insert

    eGFP-2A-mKv1.2 (Kcna2 Synthetic, Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human SYN1

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLJM1-FLAG-Sestrin2-L261A

    Plasmid

    #73676

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L261A changed Leucine 261 to Alanine

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

39. N-GFP-Dcp2

    Plasmid

    #163585

    Purpose

    express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    DCP2 promoter

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TRPML2-MYC

    Plasmid

    #18829

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML2 (Mcoln2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

41. pMC-1-PvUbi2-6

    Plasmid

    #197706

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    PvUbi2 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMC-1-AtUbi10-6

    Plasmid

    #197700

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtUbi10 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. FRB-polySH35

    Plasmid

    #175234

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. bbCas9pluspAAA

    Plasmid

    #82581

    Purpose

    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

    Depositor

    Ivo Huijbers

    Article

    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Dronpa-Vinculin-23

    Plasmid

    #57308

    Purpose

    Localization: Focal Adhesions, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Article

    Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    Dronpa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

46. pJEC625

    Plasmid

    #174368

    Purpose

    Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCR1003

    Plasmid

    #71821

    Purpose

    Express Streptococcus pyogenes dCas9 (D10A, H840A)

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

    Plasmid

    #13006

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 2 deltaCC (SULF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 88 to Alanine

    Promoter

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

49. pS2-FLAG-Sestrin2-L261A

    Plasmid

    #73678

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L261A changed Leucine 261 to Alanine

    Promoter

    Sesn2

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

50. pCfB8380

    Plasmid

    #126914

    Purpose

    Plasmid containing integration cassette at XII-5 integration site in Saccharomyces cerevisiae for overexpression of XdCrtE

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtE
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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