We narrowed to 7,280 results for: 11

1. pJRH-1363 LV EF1a-CD19 IRES-EGFP

   Plasmid

   #201919

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD19 (CD19 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 NPY pHLeon

   Plasmid

   #235176

   Purpose

   Plasmid expressing NPY tag with pH sensor, pHLeon (pH-Lemon with mNeonGreen), in mammalian cells

   Depositor

   Kate White

   Article

   Lin et al ACS Sens. 2024 Nov 22;9(11):6032-6039. d

   Insert

   NPY-pHLeon (NPY Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK1_008

   Plasmid

   #123671

   Purpose

   Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector LS-2xHS4
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MTK1_009

   Plasmid

   #123672

   Purpose

   Encodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector L1-2xHS4
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK1_013

   Plasmid

   #123676

   Purpose

   Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector L5-2xHS4
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

   Plasmid

   #190162

   Purpose

   Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

   Insert

   mcherry_(3'UTR, No Actin)_24Broccoli
   

   Tags

   24×Broccoli

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB-mTSPAN4-mCherry

   Plasmid

   #210330

   Purpose

   Overexpression of mTSPAN4

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   mTSPAN4 (Tspan4 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-TORCAR(T/A)

   Plasmid

   #64928

   Purpose

   Negative-control mutant for TORCAR biosensor.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR(T/A) (EIF4EBP1 Human, Synthetic)
   

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Mutation

   4EBP1 domain contains T37A and T46A mutations.

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-mTSPAN4-GFP

   Plasmid

   #210329

   Purpose

   Overexpression of mTSPAN4

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   mTSPAN4 (Tspan4 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Expression

    Mammalian

    Promoter

    CAG and Human U6

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-HA-JMJD3

    Plasmid

    #24167

    Purpose

    Mammalian expression of human JMJD3 with HA tag

    Depositor

    Kristian Helin

    Article

    Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

    Insert

    JMJD3 (KDM6B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. emiRFP670-cGAS

    Plasmid

    #231896

    Purpose

    Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    cGAS (CGAS Human)
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-cGAS-HA

    Plasmid

    #231895

    Purpose

    Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    cGAS (CGAS Human)
    

    Tags

    3xHA and mCherry

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-synPCB2.1

    Plasmid

    #139478

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

    Plasmid

    #211687

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-Puro

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and guide RNA recipient vector
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPUR-mU6-sgRNA-2XPP7

    Plasmid

    #194504

    Purpose

    Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.3/V5-DEST-GFP

    Plasmid

    #40125

    Depositor

    Lynda Chin

    Article

    Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3_cAMPFIRE-M

    Plasmid

    #182280

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. hSTARR-seq_ORI vector

    Plasmid

    #99296

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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