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pFTK008

Plasmid

#171280

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Poat1 Pc18g03600 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK007

Plasmid

#171279

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PfraA An16g04690 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK005

Plasmid

#171277

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Ptef1 ANIA_04218 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK003

Plasmid

#171275

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Patp9 An04g08190 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK002

Plasmid

#171274

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PgndA An11g02040 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK001

Plasmid

#171273

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Pact Pc20g11630 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pPD151 ZF37i

Plasmid

#138778

Purpose

Constitutive expression of ZF37i under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF37i

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK103

Plasmid

#110582

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Lac promoter with lac operator and RBS

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMM0621

Plasmid

#128996

Purpose

pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pFH101

Plasmid

#128206

Purpose

sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pVL37 - pWOX4

Plasmid

#116024

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Thomas Greb

Publication

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pWOX4

Use

Unspecified

Mutation

T1473A by SDM to abolish Eco31I site

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-G418R (01-21)

Plasmid

#101703

Purpose

G418R in domestication vector

Depositor

Elena Caro Bernat

Publication

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[G418R (loxP-PAgTEF-kanMX-TAgTEF-loxP)]-CGCT

Use

Yeast expression

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YCe1901 HC_Kan_PGKpA_p22

Plasmid

#100683

Purpose

part designed to occupy position 22 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

PGKpA

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDIRECT_25G

Plasmid

#91144

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-dead_3

Plasmid

#91167

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(PLF)-HA no-pA

Plasmid

#82597

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

K2095P, T2098L, W2101F

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3036(XI-1 MarkerFree)

Plasmid

#73274

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3037(XI-5 MarkerFree)

Plasmid

#73277

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE3

Plasmid

#79442

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII

Depositor

Johannes Stuttmann

Publication

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE64

Plasmid

#79446

Purpose

recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII

Depositor

Johannes Stuttmann

Publication

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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