We narrowed to 511 results for: HMG

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pRK5-HA-Ubiquitin-K29R

Plasmid

#17602

Purpose

Mammalian expression of HA tagged ubiquitin K29R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K29R; V5 deleted

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-KO

Plasmid

#17603

Purpose

Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

KO: all lysines mutated to arginines

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pIRES-Puro-MGMT

Plasmid

#187654

Purpose

Expresses hMGMT together with the puromycin resistance gene via an IRES.

Depositor

Robert Sobol

Article

Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

Insert

MGMT (MGMT Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_Ubiquitin

Plasmid

#131258

Purpose

Mammalian expression of ubiquitin with an N-terminal HA tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Promoter

CMV enhancer + CMV promoter

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_N_NFIB

Plasmid

#167783

Purpose

Expresses NFIB in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

NFIB (NFIB Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48R

Plasmid

#17604

Purpose

Mammalian expression of HA tagged ubiquitin K48R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48R (expected to disrupt G76-K48 ubiquitin chain…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pGEN25-Ub Hs

Plasmid

#83232

Purpose

pHis-par2-Ub Hs

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Ubiqutin (UBC Human)

Tags

His

Expression

Bacterial

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO UBC

Plasmid

#68456

Purpose

TOPO construct expressing UBC for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Ubiquitin C Promoter (UBC Human)

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Ubc-c005

Plasmid

#216876

Purpose

Bacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion

Depositor

Lari Lehtiö

Article

Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

Insert

Ubiquitin (UBC Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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Ubc-c004

Plasmid

#216875

Purpose

Bacterial expression vector for E. coli producing ubiquitin with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

Insert

Ubiquitin (UBC Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-pcr4

Plasmid

#193662

Purpose

Expression of tandem pre-sgRNA array pcr4 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Ubiquitin-K11R

Plasmid

#121154

Purpose

Mammalian expression of HA tagged ubiquitin K11R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K11 mutated to an R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K27R

Plasmid

#121155

Purpose

Mammalian expression of HA tagged ubiquitin K27R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K27 mutated to an R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Ubiquitin-K6R

Plasmid

#121153

Purpose

Mammalian expression of HA tagged ubiquitin K6R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K6 mutated to an R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48

Plasmid

#17605

Purpose

Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Ubiquitin-K6

Plasmid

#121151

Purpose

Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

All Ks apart from K6 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-(HA)3-ubiquitin

Plasmid

#196991

Purpose

Expresses HA-tagged ubiquitin in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

ubiquitin (UBC Human)

Tags

triple HA tag

Expression

Mammalian

Mutation

silent mutation in ubiquitin codon 13

Promoter

CMV

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-ubiquitin

Plasmid

#196999

Purpose

Expression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

ubiquitin (UBC Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K27R-K29R

Plasmid

#121156

Purpose

Mammalian expression of HA tagged ubiquitin K27R and K29R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K27 and K29 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPM6 gRNA (BRDN0001145361)

Plasmid

#77335

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM6 (TRPM6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPM6 gRNA (BRDN0001148109)

Plasmid

#77336

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM6 (TRPM6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPM6 gRNA (BRDN0001145550)

Plasmid

#77337

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM6 (TRPM6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPM6 gRNA (BRDN0001144989)

Plasmid

#77334

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM6 (TRPM6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TRIPZ-bioGEF-UBwt-PURO

Plasmid

#208045

Purpose

Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

Ub (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Ubiquitin-K11

Plasmid

#121152

Purpose

Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

All Ks apart from K11 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0406

Plasmid

#143023

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CTCFL (CTCFL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0404

Plasmid

#141606

Purpose

Lentiviral vector for overexpressing the CTCFL transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CTCFL (CTCFL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0405

Plasmid

#143212

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CTCFL (CTCFL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge