We narrowed to 2,466 results for: crispr system
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Plasmid#182208PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegyptiDepositorInsert3xP3-GFP
UseCRISPRExpressionInsectPromoterU6Available SinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-sgCDKN1B#2 BFP
Plasmid#60905PurposeA lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFPDepositorInsertBFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable SinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonorTp
Plasmid#211788PurposepDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpRDepositorInsertdhfr
Available SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEcCas6
Plasmid#170091Purposeexpresses E. coli type I-E Cas6DepositorInsertE. coli type I-E Cas6
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP2367
Plasmid#160076PurposeZ. mobilis CRISPRi, promoter C, empty guide, sfGFPDepositorInsertempty sgRNA
ExpressionBacterialAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEcCas5
Plasmid#170090Purposeexpresses E. coli type I-E Cas5DepositorInsertE. coli type I-E Cas5
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas7
Plasmid#170092Purposeexpresses E. coli type I-E Cas7DepositorInsertE. coli type I-E Cas7
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas8
Plasmid#170093Purposeexpresses E. coli type I-E Cas8DepositorInsertE. coli type I-E Cas8
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCse2
Plasmid#170094Purposeexpresses E. coli type I-E Cse2DepositorInsertE. coli type I-E Cse2
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP2373
Plasmid#160079PurposeZ. mobilis CRISPRi, promoter F, empty guide, sfGFPDepositorInsertempty sgRNA
ExpressionBacterialAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2371
Plasmid#160078PurposeZ. mobilis CRISPRi, promoter E, empty guide, sfGFPDepositorInsertempty sgRNA
ExpressionBacterialAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2369
Plasmid#160077PurposeZ. mobilis CRISPRi, promoter D, empty guide, sfGFPDepositorInsertempty sgRNA
ExpressionBacterialAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2093
Plasmid#160074PurposeZ. mobilis CRISPRi, promoter B, empty guide, sfGFPDepositorInsertempty sgRNA
ExpressionBacterialAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
nCas9-SpRY_P2A-mCherry
Plasmid#219935PurposeMammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systemsDepositorInsertSp-nCas9(D10A)-SpRY_P2A-mCherry
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationnCas9(D10A)PromoterCMVAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2R3z-AtU3b-tRNA-ccdB-gRNA
Plasmid#118390PurposeEntry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertAtU3b promoter
UseCRISPRAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
cr3Dd
Plasmid#184862Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination systemDepositorInsertCas9, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
cr3Vn
Plasmid#184861Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination systemDepositorInsertCas9, gam, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
cr3Ec
Plasmid#184860Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination systemDepositorInsertCas9, gam, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
nCas9-NG_P2A-mCherry
Plasmid#219934PurposeMammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systemsDepositorInsertSp-nCas9(D10A)-NG_P2A-mCherry
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationnCas9(D10A)PromoterCMVAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUS_A8
Plasmid#99882PurposeRecipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.DepositorTypeEmpty backboneUseTALENAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-SpCas9-P2A-blast
Plasmid#106477Purposesingle-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterEFSAvailable SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGE-AtFIE
Plasmid#246808PurposeA GreenGate entry vector containing coding region of AtFIEDepositorInsertAtFIE (FIE Mustard Weed)
UseGreengate cloning entry vectorAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGGD-AtSUC2 Promoter-scFV-sfGFP
Plasmid#246801PurposeA GreenGate entry vector containing scFV-sfGFP fusion gene drived by an AtSUC2 PromoterDepositorInsertAtSUC2 Promoter-scFV-sfGFP
UseGreengate cloning entry vectorAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
iCas
Plasmid#84232PurposeExpression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)DepositorInsertCas9
Tags2A-OFP co-expression and ERT2-ERT2ExpressionMammalianPromoterCMVAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro
Plasmid#42333PurposeThis plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEL033
Plasmid#137902PurposeSTU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editingDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas6
Plasmid#126485PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas6
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMV and U6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas8b2
Plasmid#126482PurposeEncodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas8b2
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMV and U6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas7
Plasmid#126483PurposeEncodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas7
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMV and U6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas5
Plasmid#126484PurposeEncodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas5
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMV and U6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LmoCas8b2
Plasmid#126488PurposeEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoterDepositorInsertLmoCas8b2
UseCRISPR and LentiviralTagsFlag, NLSExpressionMammalianPromoterhUbCAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LmoCas7
Plasmid#126489PurposeEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoterDepositorInsertLmoCas7
UseCRISPR and LentiviralTagsFlag, NLSExpressionMammalianPromoterhUbCAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LmoCas5
Plasmid#126490PurposeEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoterDepositorInsertLmoCas5
UseCRISPR and LentiviralTagsFlag, NLSExpressionMammalianPromoterhUbCAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LmoCas6
Plasmid#126491PurposeEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoterDepositorInsertLmoCas6
UseCRISPR and LentiviralTagsFlag, NLSExpressionMammalianPromoterhUbCAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas6
Plasmid#106274PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasE
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LmoCas6-KRAB-2a-BlastR
Plasmid#126492PurposeEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoterDepositorInsertLmoCas6-KRAB-2a-BlastR
UseCRISPR and LentiviralTagsFlag, NLSExpressionMammalianPromoterhUbCAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas8e
Plasmid#106270PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasA
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCse2
Plasmid#106271PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasB
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas7
Plasmid#106272PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasC
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas5
Plasmid#106273PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasD
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas3cRh
Plasmid#133773PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
ExpressionBacterialPromoterLac (proteins), pJ23119 (crRNA)Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pM378
Plasmid#173174PurposeExpresses C9-1 gRNA-12xMBS from hU6 promoterDepositorInsertC9-1 targeting gRNA-12xMBS
UseCRISPRExpressionMammalianPromoterhU6Available SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B2.0
Plasmid#99888PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131B2.0
Plasmid#99885PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dPspCas13b-3xmRuby3-NLS
Plasmid#132405Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralExpressionMammalianMutationH133A; H1059AAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE
Plasmid#176268PurposeExpression of dCas12f-VPR-mCherry in mammalian cellsDepositorInsertdCas12f
UseLentiviralTagsHA-VPR-mCherryExpressionMammalianMutationD326A, D510APromoterPGKAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132A2.0
Plasmid#99887PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU6Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133A2.0
Plasmid#99891PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU6Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only