We narrowed to 369 results for: PUR ALPHA-1

1. PGEX-DDX21-D2+C

   Plasmid

   #179243

   Purpose

   protein purification in E. coli

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21-D2+C (DDX21 Human)
   

   Use

   Tags

   GST-flag

   Expression

   Bacterial

   Mutation

   aa396-782

   Promoter

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPB-cT3G-cERP2-RUNX2

   Plasmid

   #192926

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   RUNX2 (RUNX2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE EF1α dCas9-KRAB

   Plasmid

   #50919

   Purpose

   Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1alpha

   Available Since

   March 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE EF1α dCas9-VP64

   Plasmid

   #50918

   Purpose

   Constitutive dCas9-VP64 lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1alpha

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TFORF3247

   Plasmid

   #144723

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF1658

   Plasmid

   #142819

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF1660

   Plasmid

   #142820

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF1659

   Plasmid

   #141862

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEM705 Gs

   Plasmid

   #109350

   Purpose

   Wildtype Gαs

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   GαS (GNAS Human)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. betaTrCP mVenus mutant reporter

    Plasmid

    #192459

    Purpose

    Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

    Depositor

    Steven Cappell

    Article

    Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

    Insert

    CdC25B
    

    Use

    Lentiviral

    Tags

    SV40 NLS and mVenus

    Expression

    Mammalian

    Mutation

    aa198-338 of CDC25B. betaTrCP degron sequence (aa…

    Promoter

    EF1alpha

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRVGP-BB

    Plasmid

    #15380

    Depositor

    Stephen Smale

    Article

    Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

    Insert

    mU6-BRG/BRM shRNA (Smarca4, Smarca2 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2008

    Availability

    Academic Institutions and Nonprofits only

12. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid

    #105842

    Purpose

    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. betaTrCP mVenus reporter

    Plasmid

    #192458

    Purpose

    Fluorescent reporter for beta-TrCP activity in human cells

    Depositor

    Steven Cappell

    Article

    Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

    Insert

    CDC25B (CDC25B Human)
    

    Use

    Lentiviral

    Tags

    SV40 NLS and mVenus

    Expression

    Mammalian

    Mutation

    aa198-338

    Promoter

    EF1alpha

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMA3379

    Plasmid

    #46874

    Purpose

    A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

    Depositor

    Mikhail Alexeyev, Troy Stevens

    Article

    Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

    Insert

    soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    TRE-Tight

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV_PP1-mCitrine

    Plasmid

    #172472

    Purpose

    Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
    

    Use

    Lentiviral

    Tags

    mCitrine and prolactin signal sequence (cleaved p…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMA3431

    Plasmid

    #46876

    Purpose

    A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3 mutant R592Q

    Depositor

    Mikhail Alexeyev, Troy Stevens

    Article

    Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

    Insert

    soluble guanylate cyclase 1alpha 3 (Gucy1a3 Rat)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    R592Q

    Promoter

    TRE-Tight

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PARD3_PDZ_3

    Plasmid

    #103959

    Purpose

    Protein expression and purification of PAR3 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PARD3 (PARD3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TFORF1027

    Plasmid

    #141784

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RARA (RARA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF1457

    Plasmid

    #143501

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TFEB (TFEB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFUPI hT5-S322-Cyp

    Plasmid

    #80282

    Purpose

    Expresses human TRIM5 CypA fusion

    Depositor

    Jeremy Luban

    Article

    Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

    Insert

    TRIM5 CypA fusion (TRIM5, PPIA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge