We narrowed to 4,352 results for: hal.1

1. pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

   Plasmid

   #199457

   Purpose

   Expression of dCas13-VPR in mammalian cells

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   dCas13
   

   Use

   Lentiviral

   Tags

   VPR-T2A-HaloTag

   Expression

   Mammalian

   Mutation

   Nuclease-dead H133A and H1058A

   Promoter

   CAG

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAdEasy-hALDOA D34S

   Plasmid

   #210813

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Adenoviral

   Tags

   HA

   Expression

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Arabidopsis thaliana Atm3 d80 pET21a+

   Plasmid

   #173045

   Purpose

   Expresses AtAtm3 in E.coli

   Depositor

   Douglas Rees

   Article

   Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

   Insert

   Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   with N-terminal 80 amino acids deletion

   Promoter

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR4 CDS A. thaliana PARP2

   Plasmid

   #175810

   Purpose

   pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 2 (PARP2, AT4G02390.1) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Article

   Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

   Insert

   PARP2 (PARP2 Mustard Weed)
   

   Use

   Pentr plasmid for gateway cloning

   Tags

   none

   Expression

   Mutation

   Promoter

   none

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBa.Halo-myosin Va tail 1006-1828

   Plasmid

   #162715

   Purpose

   Mammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residues

   Depositor

   Marvin Bentley

   Article

   Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

   Insert

   Myosin Va (Myo5a Rat)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 1005 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. siRNA-resistant ABHD16A-S355A-Halo

   Plasmid

   #195169

   Purpose

   mammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ABHD16A (ABHD16A Human)
   

   Use

   Tags

   Halo tag

   Expression

   Mammalian

   Mutation

   Wobble mutations for siRNA resistance from L90 to…

   Promoter

   CMV

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C514-E47: attB5r-Halotag-nostop-attB1r

   Plasmid

   #162963

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Halotag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NanoBRET Assay Vector, BRAF RBD-HaloTag

   Plasmid

   #236879

   Purpose

   Express BRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   HaloTag (R)

   Expression

   Mammalian

   Mutation

   Ras Binding Domain

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pLV-SspB-HaloTag-TIAM1-DHPH

   Plasmid

   #176127

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBa.Halo-Kif13B tail 442-1843

    Plasmid

    #162721

    Purpose

    Mammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Kif 13B (Kif13b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT with deletion of the 441 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 3xFlag-HaloTag-EZH2 HDR - pDY053

    Plasmid

    #171108

    Purpose

    Homology directed repair plasmid for generating HaloTag-EZH2 cells

    Depositor

    Thomas Cech

    Article

    Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. siRNA-resistant ABHD16A-H189AD194A-Halo

    Plasmid

    #195168

    Purpose

    mammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    ABHD16A (ABHD16A Human)
    

    Use

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Wobble mutations for siRNA resistance from L90 to…

    Promoter

    CMV

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEntr1a-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176134

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBa.Halo-KIF13A tail 361-1749

    Plasmid

    #162720

    Purpose

    Mammalian expression of WT mouse Kif13A with deletion of the 360 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Kif 13A (Kif13a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT with deletion of the 360 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQCXIP-mCherry-Halo-YAP1

    Plasmid

    #128336

    Purpose

    Retrovirus construct to express mCherry-Halo-YAP1

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry and Halo-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LIG4 HAL-T2A-GFP-SV40pA-HAR

    Plasmid

    #225357

    Purpose

    This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the SelectRepair knockout GFP approach.

    Depositor

    Sujatha Jagannathan

    Article

    Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

    Insert

    T2A-GFP-SV40pA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LIG4 HAL-T2A-HYG-SV40pA-HAR

    Plasmid

    #225358

    Purpose

    This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the hygromycin B SelectRepair knockout approach.

    Depositor

    Sujatha Jagannathan

    Article

    Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

    Insert

    T2A-HYG-SV40pA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LIG4 HAL-T2A-NEO-SV40pA-HAR

    Plasmid

    #225359

    Purpose

    This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the neomycin SelectRepair knockout approach.

    Depositor

    Sujatha Jagannathan

    Article

    Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

    Insert

    T2A-NEO-SV40pA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. plx208-CMV-Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit

    Plasmid

    #213538

    Purpose

    Expresses the CapC-caged opioid peptide in mammalian cells

    Depositor

    Wenjing Wang

    Article

    Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

    Insert

    Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX6P1-human LIC1 C-terminal half

    Plasmid

    #74599

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    

    Use

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    amino acids 389-523 of LIC1

    Promoter

    T7

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge