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Showing: 301 - 320 of 348 results

  1. PB-SAM-Dest

    Plasmid
    #102563
    Purpose
    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    N/A
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102563-plasmid-map-sequence-id-193893

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  4. pMDC32B-AtMIR390a-B/c

    Plasmid
    #51776
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtMIR390a-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51776-plasmid-map-sequence-id-80341

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  5. pMDC32B-AtTAS1c-B/c

    Plasmid
    #51773
    Purpose
    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51773-plasmid-map-sequence-id-80112

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  10. Equalizer-L episome

    Plasmid
    #169738
    Purpose
    Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169738-plasmid-map-sequence-id-334158

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  12. pMDC32B-B/c

    Plasmid
    #227963
    Purpose
    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka
    Insert
    B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227963-plasmid-map-sequence-id-454828

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  13. pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

    Plasmid
    #213401
    Purpose
    Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:
    Insert
    Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    213401-plasmid-map-sequence-id-422351

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  14. pCas3-APOBEC1

    Plasmid
    #229536
    Purpose
    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229536-plasmid-map-sequence-id-458911

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  15. pCas3-CDA1

    Plasmid
    #229534
    Purpose
    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229534-plasmid-map-sequence-id-458891

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  16. lentiGuide-chr20-49345720-gRNA1

    Plasmid
    #232623
    Purpose
    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.
    Depositor
    Claes Wadelius
    Article
    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1
    Insert
    S. pyogenes sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    April 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232623-plasmid-map-sequence-id-465706

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  17. lentiGuide-chr20-49345720-gRNA2

    Plasmid
    #232624
    Purpose
    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.
    Depositor
    Claes Wadelius
    Article
    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1
    Insert
    S. pyogenes sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    April 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232624-plasmid-map-sequence-id-465707

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  18. lentiGuide-chr20-49345720-gRNA3

    Plasmid
    #232625
    Purpose
    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.
    Depositor
    Claes Wadelius
    Article
    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1
    Insert
    S. pyogenes sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    April 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232625-plasmid-map-sequence-id-465708

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Showing: 301 - 320 of 348 results