We narrowed to 9,366 results for: bli

1. mDC-STAMP-Flag-Myc-CMV26

   Plasmid

   #73583

   Purpose

   Express mouse DC-STAMP in mammalian cells

   Depositor

   Paul Odgren

   Insert

   DC-STAMP (Dcstamp Mouse)
   

   Tags

   3x Flag and c-Myc tag

   Expression

   Mammalian

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1D V5-His-TOPO-HuR.wt

   Plasmid

   #110417

   Purpose

   Mammalian expression of HuR (wild type)

   Depositor

   Sandra Martha Gomes Dias

   Insert

   HuR (ELAVL1) (ELAVL1 Human)
   

   Tags

   6xHis and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX307-FURIN-blast

   Plasmid

   #158461

   Purpose

   Constitutive lentiviral expression of human FURIN.

   Depositor

   Sho Iketani, Alejandro Chavez

   Insert

   FURIN (FURIN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. BRD2

   Plasmid

   #39074

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BRD2 (BRD2 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pAC-BETAipi-ispA/dxs

   Plasmid

   #178302

   Purpose

   Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Inserts

   ispA
   dxs
   

   Expression

   Bacterial

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EGFP-hChibby1

   Plasmid

   #89770

   Purpose

   Labels mother centrioles/basal bodies

   Depositor

   Ken-Ichi Takemaru

   Insert

   Chibby1 (CBY1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   EF1alpha

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AICSDP-82:SON-mEGFP

   Plasmid

   #133964

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human SON

   Depositor

   Allen Institute for Cell Science

   Insert

   SON Homology Arms with mEGFP-linker (SON Human)
   

   Use

   CRISPR

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Nov. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLenti-mIFT88-EGFP

   Plasmid

   #149697

   Purpose

   Expresses mouse IFT88-EGFP in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Insert

   IFT88 (Ift88 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   EF1 alpha

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CTNNB1 in pENTR

   Plasmid

   #216617

   Purpose

   gateway cloning donor vector containing CTNNB1

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Gateway entry plasmid

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-Flex-Axon-GCaMP7b

    Plasmid

    #135419

    Purpose

    Can be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.

    Depositor

    Rylan Larsen

    Insert

    axon-jGCaMP7b
    

    Use

    AAV

    Tags

    6xHis and GAP43 palmitoylation domain

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQdCas12a.luxR(mut)-sggfp(C)

    Plasmid

    #236043

    Purpose

    The plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    

    Use

    CRISPR

    Promoter

    quorum sensing promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-DIO-CAG-TVA-P2A-dTomato

    Plasmid

    #177016

    Purpose

    For expression of the avian TVA receptor in a cre-dependent manner

    Depositor

    Peter Jonas

    Insert

    TVA-P2A-dTomato (CD320 Coturnix japonica)
    

    Use

    AAV and Cre/Lox

    Tags

    dTomato

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hs.NRAS G13D

    Plasmid

    #83177

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G13D mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G13D

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOET5 GII.4 Sydney noro-VLP

    Plasmid

    #218092

    Purpose

    Forms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).

    Depositor

    Minna Hankaniemi

    Insert

    noro-VLP
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-BDNF-pHluorin

    Plasmid

    #187193

    Purpose

    To express the mouse BDNF tagged with the FP pHluorin

    Depositor

    Pantelis Tsoulfas

    Insert

    BDNF (Bdnf Mouse)
    

    Use

    Lentiviral

    Tags

    pPHluorin

    Expression

    Mammalian

    Mutation

    The codon were optimized for better expression

    Promoter

    CMV

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-hACE2-cMYC-Flag

    Plasmid

    #158957

    Purpose

    Used for expression of human ACE2 with AAV packaging

    Depositor

    Akiko Iwasaki

    Insert

    Human ACE2 (ACE2 Human)
    

    Use

    AAV

    Tags

    Flag and MYC

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164

    Plasmid

    #122966

    Purpose

    Expresses 3XFlag-tagged human CEP164 in mammalian cells

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Insert

    CEP164 (CEP164 Human)
    

    Use

    Lentiviral

    Tags

    3XFlag

    Promoter

    EF1 alpha

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-dCas9-G9a-Hygro

    Plasmid

    #104408

    Purpose

    transient expression of dCas9-G9a fusion protein

    Depositor

    Stephan Beck

    Insert

    G9a (EHMT2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. LGmCreER (self deleting)

    Plasmid

    #33340

    Depositor

    Scott Lowe

    Article

    Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

    Insert

    CreERT2
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. CAMK2A

    Plasmid

    #38971

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CAMK2A (CAMK2A Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

21. Hs.HRAS C186S

    Plasmid

    #83189

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), C186S mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    C186S

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFRT-TODestGFP_HuD

    Plasmid

    #65763

    Purpose

    expresses GFP-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid

    #164637

    Purpose

    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

    Depositor

    Günter Schneider

    Insert

    target guide sequence PRMT5 inhibition (PRMT5 Human)
    

    Use

    CRISPR and Lentiviral; Interference

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET-Sac-Abeta(MC1-40)

    Plasmid

    #127152

    Purpose

    Expresses N-terminal cysteine Abeta(1-40)

    Depositor

    James Nowick

    Insert

    Abeta(MC1-40) (APP Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. XLone-BSD SARS-CoV2 N P2A mCherry

    Plasmid

    #154398

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SARS-CoV2 N Protein (N SARS-CoV2)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pFRT-TODestRFP_HuD

    Plasmid

    #65766

    Purpose

    expresses RFP-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Expression

    Mammalian

    Mutation

    V23M mutation in RFP

    Promoter

    CMV, 2x Tet

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

27. RHPMP132 (mEOS3.1-beta Actin)

    Plasmid

    #88836

    Purpose

    Localization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1

    Depositor

    Ricardo Henriques

    Insert

    beta-Actin (ACTB Human)
    

    Tags

    mEOS3.1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pDONR221-APN

    Plasmid

    #158440

    Purpose

    Gateway Cloning compatible entry vector for the human APN gene.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    APN (ANPEP Human)
    

    Use

    Gateway entry vector

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. RHPMP133 (mOrange2-beta Actin)

    Plasmid

    #88837

    Purpose

    Localization by Super-Resolution microscopy of beta-Actin fused to mOrange2

    Depositor

    Ricardo Henriques

    Insert

    beta-Actin (ACTB Human)
    

    Tags

    mOrange2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMSCV-Flag-Mm Ezh2 Y641F

    Plasmid

    #80077

    Purpose

    Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection

    Depositor

    Phillip Abbosh

    Inserts

    Flag-EZH2 Y641F (Ezh2 Mouse)
    Hygromycin phosphotransferase
    

    Use

    Retroviral

    Tags

    5' FLAG

    Expression

    Mammalian

    Mutation

    Y641F, R342R, N393N

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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31. pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

    Plasmid

    #225433

    Purpose

    Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

    Depositor

    Diego Orzaez

    Insert

    4xCBS:mDFR:Rep/RepA:Tnos
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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32. GPX3

    Plasmid

    #38899

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GPX3 (GPX3 Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pFRT-DestFLAGHA_HuB

    Plasmid

    #65758

    Purpose

    constitutively expresses FLAG-HA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pFRT-TODestGFP_HuB

    Plasmid

    #65761

    Purpose

    expresses GFP-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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35. pAAV-UbC-eGFP-F

    Plasmid

    #71545

    Purpose

    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

    Depositor

    Pantelis Tsoulfas

    Insert

    eGFP-F
    

    Use

    AAV

    Tags

    contains 20- amino-acid farnesylation signal from…

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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36. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid

    #41878

    Depositor

    Anna Huttenlocher

    Insert

    photoactivatable Rac1 (RAC1 Human)
    

    Use

    Zebrafish expression

    Tags

    6x His and mCherry

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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37. pNIC28-hKdm2b-CxxC-PHD

    Plasmid

    #49216

    Purpose

    Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.

    Depositor

    Rob Klose

    Insert

    Kdm2b (KDM2B Human)
    

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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38. COVID-SARS2 NSP16/10

    Plasmid

    #159615

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

    Depositor

    Opher Gileadi

    Inserts

    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    

    Tags

    His6-Zb-TEV and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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39. BASIC_SEVA_17_pKD46.10

    Plasmid

    #178917

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Available Since

    Feb. 2, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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40. pET21a-trAtCCD8(pYL735)

    Plasmid

    #178286

    Purpose

    Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    trAtCCD8
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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