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Showing: 7951 - 8000 of 167150 results

  1. mScarlet-I-T2A-LSS-SGFP2

    Plasmid
    #112946
    Purpose
    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
    Depositor
    Dorus Gadella
    Insert
    mScarlet-I and LSS-SGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112946-plasmid-map-sequence-id-223584

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  2. mVenus-ZO1-C-14

    Plasmid
    #56394
    Purpose
    Localization: Tight Junctions, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. L4271

    Plasmid
    #1646
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    RNAi
    Tags
    T3p, gfpN, gfpS65S, gfpC, unc2217896, unc223827, …
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1646-plasmid-map-sequence-id-256

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  5. mU6-sgLkb1_1st-hU6-sgNT

    Plasmid
    #177220
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_1st/sgNT1
    Use
    Lentiviral
    Promoter
    mU6/hU6
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177220-plasmid-map-sequence-id-350037

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  6. L4667

    Plasmid
    #1669
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, IVSA, rf2128, ATG-MitLS, gfpN…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1669-plasmid-map-sequence-id-279

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  7. p132

    Plasmid
    #17668
    Depositor
    Cecilia Lo
    Insert
    Cx43 (Gja1 Mouse)
    Expression
    Bacterial
    Available Since
    March 27, 2008
    Availability
    Academic Institutions and Nonprofits only
    17668-plasmid-map-sequence-id-8704

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  9. pSB4A3-PBAD-phzH

    Plasmid
    #165618
    Purpose
    Arabinose-inducible expression of phzH for conversion of phenazine-1-carboxylic acid to phanzine-1-carboxamide
    Depositor
    Martin Buck
    Insert
    phzH (phzH Pseudomonas aeruginosa)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    165618-plasmid-map-sequence-id-331404

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  11. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

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  12. R777-E284 Hs.ROCK1-nostop

    Plasmid
    #70568
    Purpose
    Gateway ORF clone of human ROCK1 [NM_005406.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK1 (ROCK1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70568-plasmid-map-sequence-id-126017

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  13. EGFP-PKCZdel239

    Plasmid
    #110513
    Purpose
    Constitutive Active
    Depositor
    Bernardo Mainou
    Insert
    PKCZ (PRKCZ Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deleted amino terminal 239 amino acids
    Available Since
    July 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110513-plasmid-map-sequence-id-210749

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  14. pY123 (pcDNA3.1-huCMaCpf1)

    Plasmid
    #92299
    Purpose
    Expression of humanized CMaCpf1
    Depositor
    Feng Zhang
    Insert
    huCMaCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92299-plasmid-map-sequence-id-181633

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  15. L4043

    Plasmid
    #1624
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    hsp16-415', hsp16-25', IVSA, SV40NLS, S…
    Expression
    Worm
    Available Since
    May 25, 2005
    Availability
    Academic Institutions and Nonprofits only
    1624-plasmid-map-sequence-id-234

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  17. hSyn1-FLEX-hChR2-tdtomato

    Plasmid
    #41015
    Depositor
    Karel Svoboda
    Insert
    Channelrhodopsin-2
    Use
    Adenoviral
    Tags
    tdtomato
    Expression
    Mammalian
    Mutation
    codon-optimized (human)
    Promoter
    synapsin
    Available Since
    Nov. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  19. LHP462 - Ub-HA

    Plasmid
    #32169
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HA
    Expression
    Yeast
    Promoter
    CUP1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  21. mPlum-Paxillin-22

    Plasmid
    #55993
    Purpose
    Localization: Focal Adhesions, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    Paxillin (PXN Chicken)
    Tags
    mPlum
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pX330-Flag-eSpCas9-H840A

    Plasmid
    #80454
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80454-plasmid-map-sequence-id-151472

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  23. pSiM3

    Plasmid
    #199382
    Purpose
    Encodes dual cistronic membrane localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP7s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199382-plasmid-map-sequence-id-391043

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  25. R777-E354 Hs.TSC1-nostop

    Plasmid
    #70638
    Purpose
    Gateway ORF clone of human TSC1 [NM_000368.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    TSC1 (TSC1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70638-plasmid-map-sequence-id-126087

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  26. L4663

    Plasmid
    #1665
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, SV40NLS, gfpN, IVSR, gfpY66W,…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1665-plasmid-map-sequence-id-275

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  27. pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)

    Plasmid
    #128171
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128171-plasmid-map-sequence-id-247962

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  28. mPA-GFP-MyosinIIA-C-18

    Plasmid
    #57149
    Purpose
    Localization: Cytoskeleton, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    MyosinIIA
    Tags
    mPA-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 19, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. mVenus-tdTomato-FRET-10

    Plasmid
    #58156
    Purpose
    Localization: Cytosolic FRET, Excitation: / 554, Emission: / 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mVenus and tdTomato
    Expression
    Mammalian
    Available Since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. pAS1-his-24::tagRFP

    Plasmid
    #124329
    Purpose
    Expressing Nuclear Localized tagRFP
    Depositor
    Andrew Leifer
    Insert
    tagRFP (his-24 Nematode)
    Expression
    Worm
    Available Since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124329-plasmid-map-sequence-id-241910

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  33. tdEos-Integrin-Beta3-N-18

    Plasmid
    #57628
    Purpose
    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Integrin-Beta3
    Tags
    tdEos
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. L4640

    Plasmid
    #1662
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo2-1090, myo25', IVSA, rf2128, gfpN, IVSR,…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1662-plasmid-map-sequence-id-272

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  35. mEmerald-STAG2-C-18

    Plasmid
    #54259
    Purpose
    Localization: Chromosome Cohesion , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    STAG2 (STAG2 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. cEF1a-LSL-GFP

    Plasmid
    #135672
    Purpose
    Introduce Cre-inducible (EF1a promoter followed by lox-stop-lox) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135672-plasmid-map-sequence-id-325851

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  37. OTV-dDAT

    Plasmid
    #32812
    Depositor
    Susan Amara
    Insert
    dDAT (DAT Fly)
    Use
    Oocyte expression
    Promoter
    T7
    Available Since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only

  38. 4IGZ

    Plasmid
    #51323
    Depositor
    Cheryl Arrowsmith
    Insert
    4IGZ (SORBS2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. SnFR_S074

    Plasmid
    #182811
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    S074-CholineSnFR
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182811-plasmid-map-sequence-id-358311

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  40. pRVdG-4ChR2-mCherry

    Plasmid
    #59328
    Purpose
    Expresses ChR2-mCherry
    Depositor
    Ian Wickersham
    Insert
    ChR2-mCherry
    Use
    Rabies virus
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    CMV
    Available Since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    59328-plasmid-map-sequence-id-92024

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  41. U25m

    Plasmid
    #121027
    Purpose
    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RiboJ + UTR1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121027-plasmid-map-sequence-id-238073

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  42. pY114 (pcDNA3.1-huPgCpf1)

    Plasmid
    #92273
    Purpose
    Expression of humanized PgCpf1
    Depositor
    Feng Zhang
    Insert
    huPgCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92273-plasmid-map-sequence-id-181493

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  43. pY122 (pcDNA3.1-huCPbCpf1)

    Plasmid
    #92298
    Purpose
    Expression of humanized CPbCpf1
    Depositor
    Feng Zhang
    Insert
    huCPbCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92298-plasmid-map-sequence-id-181648

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  45. mNeptune2-PMP-N-10

    Plasmid
    #56148
    Purpose
    Localization: Peroxisomes, Excitation: 600, Emission: 650
    Depositor
    Michael Davidson
    Insert
    PMP (PXMP2 Human)
    Tags
    mNeptune2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. pOTX2-donor

    Plasmid
    #176358
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    OTX2 (OTX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176358-plasmid-map-sequence-id-347862

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  48. SRS-MTBD(89/82)-ABux

    Plasmid
    #22384
    Depositor
    Ron Vale
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Tags
    His and myc
    Expression
    Bacterial
    Available Since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22384-plasmid-map-sequence-id-10292

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  50. mEmerald-Vinculin-N-18

    Plasmid
    #54303
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    NM_003373.3 (VCL Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7951 - 8000 of 167150 results