We narrowed to 35,392 results for: CaS;

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pMS26: pTns(PmcCAST)_ΔTniQ

Plasmid

#168159

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST Tns proteins (TnsAB, TnsC and TnsD)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS27: pTns(PmcCAST)_ΔTnsD

Plasmid

#168160

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6-KRAB-2a-BlastR

Plasmid

#126492

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6-KRAB-2a-BlastR

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

Plasmid

#160610

Purpose

Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

P35s:AsCas12a:T35s

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-(BsaI)_CBh-Cas9

Plasmid

#135010

Purpose

Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

Depositor

Yosef Shaul

Article

Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

Insert

humanized S. pyogenes Cas9

Tags

3xFLAG (N terminal on insert)

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC6-Flag CASTOR1 I280A

Plasmid

#84497

Purpose

Lentiviral expression of CASTOR2 I280A mutant

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 I280A (GATSL3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

I280A

Promoter

sestrin2

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pT7-AsCas12a_crRNA-site3 (RTW549)

Plasmid

#160138

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(D977A)-2AeGFP

Plasmid

#121962

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Tags

3xHA tag

Expression

Mammalian

Mutation

D977A

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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SauCas9KKH CCR5-nicking sgRNA

Plasmid

#169863

Purpose

SauCas9KKH nicking sgRNA for CCR5

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9KKH CCR5-nicking sgRNA

Expression

Mammalian

Promoter

human U6 promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGabrg3

Plasmid

#124858

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin2a

Plasmid

#124863

Purpose

Mutagenesis of Grin2a

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2a (Grin2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

Plasmid

#124859

Purpose

Mutagenesis of Slc17a7

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a7 (Slc17a7 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGria3

Plasmid

#124869

Purpose

Mutagenesis of Gria3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria3 (Gria3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

Plasmid

#124872

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

Plasmid

#124871

Purpose

Mutagenesis of Gabrg2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg2 (Gabrg2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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CNC3G (CASANOVA-C3 with glycine linkers)

Plasmid

#137192

Purpose

Expresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory protein

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

Insert

CNC3G (CASANOVA-C3 with glycine linkers)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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PX382 Staphylococcus pseudintermedius Cas9

Plasmid

#68319

Purpose

Staphylococcus pseudintermedius Cas9

Depositor

Feng Zhang

Insert

SpsCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

Plasmid

#92433

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pD2-dcas9-J23101-GFP

Plasmid

#122260

Purpose

Constitutive dCas9 and GFP expression plasmid

Depositor

Chueh Loo Poh

Article

Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

Insert

dCas9

Use

Synthetic Biology

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CMV-TO-Casp-rastvTEVca-teTVMVd1

Plasmid

#116099

Purpose

Expresses a combination of proteases and their reporter

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Casp-rastvTEVca-teTVMVd1

Expression

Mammalian

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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