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pCMV-SpdCas9-VP64

Plasmid

#115794

Purpose

Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

VP64

Use

AAV

Tags

3XFLAG and dCas9-VP64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMMLV-EGFP-P2A-Puro

Plasmid

#160226

Purpose

Expresses EGFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EGFP-P2A-PuroR

Use

Retroviral

Expression

Bacterial and Mammalian

Promoter

LTR

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-CoV2-WT

Plasmid

#177618

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6a-V106W

Plasmid

#215824

Purpose

Express CBE6a-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6a V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP_IRES_tdTomato

Plasmid

#184046

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD(E488Q)

Plasmid

#170125

Purpose

Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD(E488Q)-NES

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a core promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-Inframe-noFSE

Plasmid

#177619

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-EGFP:Ajuba

Plasmid

#108229

Purpose

Lentiviral vector for Tet-inducible Ajuba expression

Depositor

Kenneth Irvine

Article

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

AJUBA (AJUBA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC43

Plasmid

#66565

Purpose

sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2XPP7
PCP-VP64 IRES mCherry

Expression

Mammalian

Promoter

CMV and U6

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-CreiLOV-P2A-FusionRed

Plasmid

#192818

Purpose

Expresses CreiLOV with FusionRed tag in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

CreiLOV

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pNoTag-DGCR8

Plasmid

#164694

Purpose

Express DGCR8 without tag in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

DGCR8 (DGCR8 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NES

Plasmid

#124703

Purpose

Expresses MS2_adRNA targeting the RAB7A locus along with MCP_ADAR1 DD (E1008Q)_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E1008Q)_NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

E1008Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-Flag-Rheb

Plasmid

#165031

Purpose

expression of Flag tagged Rheb protein in mammalian cells

Depositor

Ken Inoki

Article

Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

Insert

Rheb (RHEB Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_NFL(WT)-FLAG

Plasmid

#182654

Purpose

Expresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

wild type mouse neurofilament light chain (Nefl Mouse)

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES

Plasmid

#124707

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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tetO7_Ef1a_SOX2

Plasmid

#18919

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

SOX2 (SOX2 Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pMRX-IZ-ANXA5-mRuby3

Plasmid

#184891

Purpose

Stable expression of ANXA5-mRuby3 (Annexin A5-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA5 (ANXA5 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:CIBN

Plasmid

#135986

Purpose

Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the CIBN domain

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-WT

Plasmid

#177614

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pphiLOV3-N1

Plasmid

#178973

Purpose

Expresses phiLOV3 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

phiLOV3

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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