We narrowed to 10,951 results for: T7

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pET30ax

Plasmid

#85761

Purpose

A bacterial expression vector with a His-tag at N and C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

His tag (N-term and C-term)

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pET N6his GFP1-9

Plasmid

#182240

Purpose

Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

Depositor

Stéphanie Cabantous

Article

Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

Insert

GFP1-9

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-mSA-EGFP

Plasmid

#39862

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin fused to EGFP

Tags

6xHis, EGFP, and FLAG

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-L2-M3T1L

Plasmid

#33304

Depositor

Terence Dermody

Article

Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

Inserts

L2 (L2 Orthoreovirus T1L)
M3 (MoV1sM3gp1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pT7-L3-S3T1L

Plasmid

#33305

Depositor

Terence Dermody

Article

Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

Inserts

L3 (MoV1sL3gp1 )
S3 (MoV1sS3gp1 )

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pT7-L1-M2T1L

Plasmid

#33303

Depositor

Terence Dermody

Article

Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

Inserts

L1 (L1 Orthoreovirus T1L)
M2 (M2 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pT7-L2-M3T3D

Plasmid

#33300

Depositor

Terence Dermody

Article

Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

Inserts

L2 (L2 Orthoreovirus T3D)
M3 (M3 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pML433

Plasmid

#177190

Purpose

Expresses recombinant HyPro enzyme in E. coli

Depositor

Eugene Makeyev

Article

Yap et al Mol Cell. 2021 Nov 2. pii: S1097-2765(21

Insert

HyPro enzyme

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CylLL_CylM

Plasmid

#208759

Purpose

Expresses modified cytolysin L (mCylLL) in E. coli

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CylLL
CylLM

Tags

Hisx6 Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaA

Plasmid

#50386

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

Depositor

Erick Strauss

Article

Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

Insert

PanK

Tags

6XHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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His-lsMSP1E3D1

Plasmid

#240152

Purpose

Expression of the MSP 'His-lsMSP1E3D1'

Depositor

Lise Arleth

Article

Tidemand et al Biochim Biophys Acta Biomembr. 2022 Jun

Insert

His-lsMSP1E3D1

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-Simple-gRNA backbone

Plasmid

#51306

Purpose

gRNA expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Type

Empty backbone

Use

CRISPR; Xenopus expression

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pACYC-GFPM2

Plasmid

#102791

Purpose

Expresses GFP under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLysscys255

Plasmid

#36429

Depositor

Emine Gunhan, Pete Lollar

Article

Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

Insert

Saporin-3/cys255

Expression

Bacterial

Mutation

cys added at position 255

Promoter

T7

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P-R

Plasmid

#189849

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P (T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huMb2Cas12a

Plasmid

#174676

Purpose

Expresses 6xHis-MBP tagged Mb2Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huMb2Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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