We narrowed to 4,337 results for: U6 gRNA

1. pEn-C1.1

   Plasmid

   #61479

   Purpose

   Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

   Depositor

   Holger Puchta

   Article

   Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

   Insert

   AtU6-26:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6-26

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-sg-mGreenLantern/EGFP

   Plasmid

   #179913

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern/EGFP sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_186

   Plasmid

   #133458

   Purpose

   U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAf-CRISPR-phoA

   Plasmid

   #207991

   Purpose

   CRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

   Insert

   phoA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Aspergillus flavus U6

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSicoR-RNF40i1

   Plasmid

   #59594

   Purpose

   Expression of shRNA against human RNF40

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   RNF40 (RNF40 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

6. GL2 shRNA

   Plasmid

   #86083

   Purpose

   A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   control shRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 EB1 shRNA #3

   Plasmid

   #37927

   Depositor

   Torsten Wittmann

   Article

   Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

   Insert

   EB1 shRNA #3 (MAPRE1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSicoR-TTF2i2

   Plasmid

   #59623

   Purpose

   Expression of shRNA against human TTF2

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   TTF2 (TTF2 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSicoR-TTF2i1

   Plasmid

   #59622

   Purpose

   Expression of shRNA against human TTF2

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   TTF2 (TTF2 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pGEM-sgMUC4_dual-MTS-3x-Set I

    Plasmid

    #162762

    Purpose

    Separately expressing 3 unique sgMUC4_dual-MTS (SetI)

    Depositor

    Antony K Chen

    Article

    Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

    Insert

    sgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. BoxB-MS2 adRNA (RAB7A-ACG site)

    Plasmid

    #170155

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(ACG)-MS2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-sg-tdTomato90/816

    Plasmid

    #179918

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. BoxB-MS2 adRNA (RAB7A-UAG site)

    Plasmid

    #170138

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(UAG)-MS2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. px330-MRPsg1

    Plasmid

    #87189

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 1

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #1 (RMRP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX552-mScn2a-HaloTag-V5-EF1-sfGFP

    Plasmid

    #182564

    Purpose

    For mouse Nav1.2 knockin with HaloTag-V5 at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn2a gRNA and HaloTag-V5 donor DNA
    sfGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1 and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. px330-MRPsg2

    Plasmid

    #87190

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 2

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #2 (RMRP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV_A2S-8

    Plasmid

    #188654

    Purpose

    AAV vector expressing SaAID-2S and gRNA

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    AID2S--nSaCas9-UGI
    Sa-sgRNA
    

    Use

    AAV and CRISPR

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    Scp1 and U6

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEM-sgIGR_dual-MTS-3x-Set I

    Plasmid

    #162764

    Purpose

    Separately expressing 3 unique sgIGR_dual-MTS (SetI)

    Depositor

    Antony K Chen

    Article

    Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

    Insert

    sgIGR_dual-MTS_1; sgIGR_dual-MTS_2; sgIGR_dual-MTS_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GluR2_adRNA(2,100,50)-GFP

    Plasmid

    #124622

    Purpose

    Expresses adRNA(2,100,50) targeting the RAB7A transcript along with GFP

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    GluR2_adRNA(2,100,50)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GluR2_adRNA(0,100,50)-GFP

    Plasmid

    #124623

    Purpose

    Expresses adRNA(0,100,50) targeting the RAB7A transcript along with GFP

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    GluR2_adRNA(0,100,50)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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