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pLHCX-Flag-mPKM2

Plasmid

#42512

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2

Use

Retroviral

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pVk7-33G1-BioLHis

Plasmid

#58949

Purpose

backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

Depositor

Gavin Wright

Article

Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

Type

Empty backbone

Use

Tags

6His tag and biotinylation peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE666

Plasmid

#153231

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:DsRed:Tnos (GB0361)

Plasmid

#68220

Purpose

Transcriptional unit for DsRed plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

DsRed

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pAT-WT-HA-GFP11

Plasmid

#177719

Purpose

ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM55285

Plasmid

#153212

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM9037

Plasmid

#153225

Purpose

Template for PCR amplification of the constant part of guide RNAs

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

guide RNA constant part, carotenoid marker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM1

Plasmid

#42511

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M1

Use

Retroviral

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
pMOD_B2518

Plasmid

#91075

Purpose

Module B, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Tags

Expression

Mutation

Promoter

TaU6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C2906

Plasmid

#91093

Purpose

Module C, Promoter: FMV 34S, Gene: TREX2 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2 (+ BaeI site for GT donor cloning) (Trex2 Mouse)

Use

Unspecified

Tags

Expression

Mutation

Promoter

FMV 34S

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET30a-FluA-NP

Plasmid

#165092

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ262m3

Plasmid

#149556

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE670

Plasmid

#153236

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE668

Plasmid

#153233

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GW1-mito-mCherryEA

Plasmid

#207868

Purpose

Mitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

Depositor

Mathew Tantama

Article

Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

Insert

mito-mCherryEA

Use

Tags

4xCox8 mitochondrial targeting

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE667

Plasmid

#153232

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMP71-tCD19-LLO-SIINFEKL

Plasmid

#174598

Purpose

expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopes

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGWB426

Plasmid

#68691

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

No Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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35S::Cel1SP-GeNL/pCAMBIA1301

Plasmid

#190069

Purpose

Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

Depositor

Takeharu Nagai

Article

Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

Insert

Cel1 signal peptide-Green-enhanced Nano-lantern

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtMIR390a-NbSu-2

Plasmid

#213400

Purpose

Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor

Depositor

Alberto Carbonell

Article

Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

Insert

Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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