We narrowed to 29,740 results for: REP

1. USPL1cat-pEYFP_C1

   Plasmid

   #85766

   Purpose

   mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, catalytic domain (aa 212-514) (USPL1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   catalytic domain (aa 212-514)

   Promoter

   CMV

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p-mCherry-C1-Phobos

   Plasmid

   #98166

   Purpose

   blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Phobos gene
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG416GAL-ccdB-mRuby3

   Plasmid

   #166139

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG eGFP-Oct4

   Plasmid

   #203789

   Purpose

   Competitive assay of Oct4 and Oct6 binding to DNA motifs.

   Depositor

   Thorsten Wohland

   Article

   Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

   Insert

   Pou5f1 (Pou5f1 Mouse)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMTB2-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80067

   Purpose

   Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Beta-actin2

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-mCherry-C1-Aurora

   Plasmid

   #98167

   Purpose

   red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Aurora gene
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/CIP2A(1-905) R522D V5 His

   Plasmid

   #119289

   Purpose

   CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

   Depositor

   Jukka Westermarck

   Article

   Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

   Insert

   KIAA1524 (CIP2A Human)
   

   Use

   Tags

   V5 His

   Expression

   Mammalian

   Mutation

   R522D

   Promoter

   T7

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 shETV1-A

   Plasmid

   #74973

   Purpose

   shETV1 shRNA

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ETV1 (ETV1 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT-ubb-Avi-Cerulean-Rpl10a

   Plasmid

   #79882

   Purpose

   Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
   

   Use

   Tags

   Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid

    #124660

    Purpose

    reporter protein which emits bright blue fluorescence

    Depositor

    Yutaka Kuroda

    Article

    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

    Insert

    Gaussia luciferase-C9D-TGinsert
    

    Use

    Tags

    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

    Expression

    Bacterial

    Mutation

    changed glutamic acid 100 to alanine, and glycine…

    Promoter

    T7 promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1 shETV1-B

    Plasmid

    #74974

    Purpose

    shETV1 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV1 (ETV1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Use

    Tags

    Cmyc

    Expression

    Mutation

    N343Q

    Promoter

    GAL1

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA BRCA1 C64Y

    Plasmid

    #139326

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 C64Y using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgRNA BRCA1 E638K

    Plasmid

    #139327

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 E638K using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSN25

    Plasmid

    #100110

    Purpose

    expresses a chimeric SOX2-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to full length HP1 alpha (SOX2, CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

    Plasmid

    #176061

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSicoR-sh4EBP2

    Plasmid

    #81121

    Purpose

    expresses an shRNA targeting murine 4E-BP2

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    4E-BP2 shRNA (Eif4ebp2 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

    Plasmid

    #51255

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Ala

    Promoter

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pKI-cjFOXP2-hum

    Plasmid

    #129757

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    FOXP2 (LOC100411307 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    T301N/N323S

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid

    #114143

    Purpose

    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80060

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p-mCherry-C1-iC++

    Plasmid

    #98165

    Purpose

    artificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iC++ gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, V242R, T24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNHT7::RPL15::hGluc

    Plasmid

    #186768

    Purpose

    An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Gaussia luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcmv3-IRG1-H159Q-HA

    Plasmid

    #198183

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1) H159Q

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The histidine in IRG1 at position 159 mutates to …

    Promoter

    CMV

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAC8_PH-GST-Nter-GWs-Lox (VE5586)

    Plasmid

    #163766

    Purpose

    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal GST tag
    

    Use

    Tags

    GST Tag

    Expression

    Insect

    Mutation

    Promoter

    PH

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

    Plasmid

    #189635

    Purpose

    Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pGMC00034 (aka pMC0235)

    Plasmid

    #210751

    Purpose

    Non-targeting guide RNA

    Depositor

    Raj Chari

    Article

    Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

    Insert

    Non-targeting guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. sgRNA BRCA2 V572I

    Plasmid

    #139321

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 V572I using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 V572I using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192788

    Purpose

    aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody enhancer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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