We narrowed to 7,181 results for: RAP

1. pMZ-181

   Plasmid

   #195429

   Purpose

   Transient, constitutive expression of synZiFTR: ZF6-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pUb - 3XFLAG - NLS - ZF6-4 (ZF6) - p65 - RbGpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SBP-mCherry-Ecadherin_puromycin

   Plasmid

   #65293

   Purpose

   Ecadherin reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Use

   Tags

   IL-2 Signal Sequence and Streptavidin Binding Pro…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc

   Plasmid

   #91934

   Purpose

   Bacterial expression of maize SUMO1a and SCE1d

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   6His-ZmSUMO1a-GG
   ZmSCE1d-Myc
   

   Use

   Tags

   6His and Myc

   Expression

   Bacterial

   Mutation

   Truncation of ZmSUMO1a after the diGlycine motif

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. ManII-SBP-EGFP_puromycin

   Plasmid

   #65260

   Purpose

   ManII reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   truncated Mannosidae II
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-KDEL_flGalT-SBP-tagBFP

   Plasmid

   #65274

   Purpose

   synchronize trafficking of GalT from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   galactosidase
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDM1515

   Plasmid

   #109000

   Purpose

   safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Safe haven knock-in plasmid for the act5 locus, c…

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pADH139

   Plasmid

   #90987

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, C, mal LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK1_008

   Plasmid

   #123671

   Purpose

   Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector LS-2xHS4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMZ-432

   Plasmid

   #195463

   Purpose

   Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: ZF3-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pSFFV - SMASh - ZF3 - p65 - ERT2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMZ-431

    Plasmid

    #195462

    Purpose

    Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: ZF3-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pSFFV - ZF3 - p65 - ERT2 - SMASh
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPI458

    Plasmid

    #113212

    Purpose

    safe haven knock-in plasmid for the act5 locus, C-terminal mScarlet tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Safe haven knock-in plasmid for the act5 locus, c…

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

    Plasmid

    #133342

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOVC1_EcSSPB-nano_X

    Plasmid

    #131212

    Purpose

    Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    EcSSPB-nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME-AKT-PH

    Plasmid

    #67674

    Purpose

    Gateway entry clone containing AKT-PH with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    AKT-PH
    

    Use

    Gateway multisite middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDM1514

    Plasmid

    #108999

    Purpose

    safe haven knock-in plasmid for the act5 locus, N-terminal mCherry tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Safe haven knock-in plasmid for the act5 locus, n…

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTK5_012

    Plasmid

    #123856

    Purpose

    Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector RE'-A1 Insulator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MTK7_013

    Plasmid

    #123880

    Purpose

    Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    RA_hAAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. TOPO CCSP

    Plasmid

    #68453

    Purpose

    TOPO construct expressing CCSP for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Clara Cell Secretory Protein Promoter
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK0_030

    Plasmid

    #123969

    Purpose

    Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    phiC31-hygro-mRuby2PEST landing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP

    Plasmid

    #199357

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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